汉防己甲素对伊曲康唑、伏立康唑和泊沙康唑抗烟曲霉体外增效活性研究  

In vitro study on the synergistic effect of tetrandrine to itraconazole,voriconazole and posaconazole against Aspergillus fumigatus

作　　者：李水秀[1,2] 宋延君[1,2] 石建萍[1,2,3] 郭慧 张玲玲 张宏 

机构地区：[1]暨南大学附属第一医院 [2]暨南大学真菌病研究所,广州510632 [3]广州医学院附属深圳沙井医院皮肤科,深圳518000

出　　处：《中国人兽共患病学报》2015年第5期408-411,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(81171542/81471995)~~

摘　　要：目的探讨汉防己甲素(tetrandrine,TET)对伊曲康唑(itraconazole,ITR)、伏立康唑(voriconazole,VRC)和泊沙康唑(posaconazole,PCZ)体外抗烟曲霉有无增效活性。方法参照M38-A2方案的微量稀释法,以23株烟曲霉临床株为研究对象,测定TET对其的最大非细胞毒性剂量和ITC、VRC、PCZ单独及联合TET对其的最小抑菌浓度(minimal inhibitory concentrations,MICs)。结果 TET抗烟曲霉的最大非细胞毒性剂量为80μg/mL。ITC单独及联合TET对烟曲霉的MIC值范围分别为0.5μg/mL^32μg/mL和0.0625μg/mL^4μg/mL,差异有统计学意义(P<0.05);VRC单独及联合TET的MIC值范围分别为0.25μg/mL^2μg/mL和0.03125μg/mL^0.25μg/mL,差异有统计学意义(P<0.05);PCZ单独及联合TET的MIC值范围分别为0.125μg/mL^1μg/mL和0.03125μg/mL^0.25μg/mL,差异有统计学意义(P<0.05)。结论汉防己甲素在体外对伊曲康唑、伏立康唑和泊沙康唑抗烟曲霉有增效活性。The purpose of this study is to investigate the synergistic effect of itraconazole(ITR),voriconazole(VRC)or posaconazole(PCZ)alone and in combination with tetrandrine(TET)against Aspergillus fumigatus.We investigated the in vitro antifungal effects of ITR/VRC/PCZ alone and in combination with TET against 23 clinical isolates of A.fumigatus using the microdilution method described in M38-A.The minimum inhibitory concentrations(MIC s)of ITR alone and combined with TET against the A.fumigatus were 0.5-32μg/mL and 0.0625-4μg/mL,respectively(P<0.05);the MIC s of VRC alone and combined with TET against the A.fumigatus were 0.25-2μg/mL and 0.031 25-0.25μg/mL,respectively(P<0.05);the MIC s of PCZ alone and combined with TET against the A.fumigatus were 0.125-1μg/mL and 0.031 25-0.25μg/mL,respectively(P<0.05).Thus,TET acts synergistically with ITR/VRC/PCZ against A.fumigatus in vitro.

关 键 词：汉防己甲素 伊曲康唑 伏立康唑 泊沙康唑 烟曲霉 增效作用 

分 类 号：R519[医药卫生—内科学]