疟疾感染过程中Tregs对DCs免疫功能的抑制作用与机理  

CD4^+ CD25^+ Foxp3^+ regulatory T cells inhibition for the immunologic function of DCs in malaria

作　　者：陈光[1] 刘蕾[1] 王芳芳 罗兰 苏菊香[1] 蔡连顺[1] 代月[1] 

机构地区：[1]佳木斯大学基础医学院,佳木斯154000 [2]佳木斯大学附属第一医院,佳木斯154000

出　　处：《中国人兽共患病学报》2015年第5期427-431,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.81101278);黑龙江省自然科学基金(No.LC2013C34);黑龙江省教育厅海外学人资助项目(No.1254HQ017)~~

摘　　要：目的探讨疟疾感染过程中调节性T细胞(Treg)对DCs免疫功能的抑制作用与机理。方法建立P.y17XL感染和Tregs消除BALB/c小鼠模型,计数红细胞感染率;感染后第0、3和5d制备脾细胞悬液,FACS检测脾脏中DCs亚群数量变化;DCs表面分子MHC-II、CD80和CD86的表达水平及分泌IL-10的DCs数量变化。结果与正常感染组小鼠相比,Tregs消除组小鼠于感染后第3dDCs亚群,MHC-II,CD80和CD86的表达均明显增加,感染后第5dDCs亚群数量、MHC-II和CD80表面分子的表达均明显减少;然而分泌IL-10的DCs数量于感染后第5d明显增加,是同天感染鼠的3.5倍。另外,我们采用两种anti-CD25mAb体内阻断Tregs,效果有着明显的差异。7D4能长期有效的阻断CD25表达,而PC61仅能短期内维持CD25低表达。结论 P.y17XL感染早期,BALB/c小鼠Tregs数量升高与DCs功能受损具有相关性。Tregs能抑制DCs的免疫功能,这一现象可能与Tregs调控DCs的亚群、表型和细胞因子分泌模式等方面有关。To investigate the role of Tregs on inhibit the immunologic function of DCs in malaria,BALB/c mice with normal and CD25 blocking were infected by intraperitoneal injection with 1 106 virulent P.y17 XL parasitized erythrocytes,and the ratio of infected RBC was counted.On day 0,3and 5post infection(pi),splenocyte the percentage of DC1/DC2,the levels of MHC-II and CD80 expression on DCs,and the percentage of DC-secreting-IL-10 were dynamically detected by FACS.Results showed that the levels of MHC-II and CD80 expression on DCs increased on day 3pi in CD25-depleted mice compared with normal infection mice;however,on day 5pi,the numbers of DC1/DC2,the levels of MHC-II and CD80 expression on DCs obviously decreased in CD25-depleted BALB/c mice.Moreover,we also found that the numbers of DC-secreting-IL-10 increased rapidly on day 5pi in CD25-depleted BALB/c mice,which was approximately one time(7D4)/3.5times(PC61)that in normal infection mice at detected time points.In addition,we used two kinds of anti-CD25 mAb to deplete Tregs in vivo,the effect is obviously different.The 7D4 mAb could long-term depleted the expression of CD25;however PC61 mAb just maintained the lower expression of CD25 for a short moment.These results indicate that the increase of Tregs amount in Py17 XL infected mice is closely correlated with impaired function of DCs in the early stage of infection.Tregs inhibited the immunologic function of DCs,maybe related to Tregs regulating the subsets,phenotype and cytokine secretion pattern of DCs.

关 键 词：疟疾感染 调节性T细胞 DCS 免疫功能 

分 类 号：R531.3[医药卫生—内科学]