肾衰冲剂抑制体外肾成纤维细胞增殖及分泌TNF-α的作用  

Inhibitiory Effects of Shenshuai Instant Granules on the Proliferation of Renal Fibroblasts and on Their TNF-α Secretion in Vitro

作　　者：高建东[1] 何立群[1] 侯卫国[1] 郑平东[1] 

机构地区：[1]上海中医药大学附属曙光医院肾内科,上海200021

出　　处：《北京中医药大学学报》2004年第1期27-30,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家中医药管理局课题 (No.95A2 15 1);"上海市医苑新星"培养计划资助

摘　　要：目的　通过观察肾衰冲剂在体外对肾成纤维细胞增殖和分泌TNF α的影响和作用 ,阐释其减轻肾纤维化的机制。方法　制备肾衰大鼠毒素血清及肾衰冲剂含药血清 ,加入成纤维细胞培养液中 ,观测细胞增殖和分泌的变化。结果　大鼠体内用药后ET 1由 (30 5 5 74± 16 6 6 5 )ng/L降至(1184 90± 191 6 4)ng/L(P <0 0 1) ;体外用药后成纤维细胞的增殖由 (0 5 34± 0 0 37)降至 (0 4 99±0 0 5 0 ) (P <0 0 5 ) ,分泌的TNF α由 (8 33± 1 4 8)IU/mL降至 (5 70± 0 6 9)IU/mL(P <0 0 1)。结论　肾衰冲剂可以抑制成纤维细胞的增殖 ,其机制之一可能与降低ET 1从而减少成纤维细胞TNFObjective By observing the effects of Shenshuai Instant Granules (SIG) on the proliferation of renal fibroblasts (PRF) and on their TNF α secretion (TNF αS) in vitro, to explain SIG's mechanism of relieving renal fibrosis. Methods Toxic serum and SIG medicated serum were prepared with the rats of renal failure. And the two kinds of serum were into the renal fibroblast culture fluid, and he changes in PRF and TNF αS were observed.Results The level of ET 1 in the rats of renal failure were decreased from 3055.74±166.65 to 1184.90±191.64 ( P <0.01) after SIG administration in vivo. PRF were decreased from 0.534±0.037 to 0.499±0.050 ( P <0.05), and TNF αS were decreased from 8.33±1.48 to 5.70±0.69 ( P <0.01) after SIG administration in vitro.Conclusion SIG can restrain PRF, one mechanism of which may be related with decreasing TNF αS of the fibroblast through lowering the level of ET I .

关 键 词：肾衰冲剂 成纤维细胞 肿瘤坏死因子-Α 

分 类 号：R259.923[医药卫生—中西医结合]