昆虫病原线虫斯氏线虫和异小杆线虫对长角血蜱雌蜱的致病力  

Virulence of entomopathogenic nematodes (Steinernematidae and Heterorhabditida e) to female ticks of Haemaphysalis longicornis Neumann (Acari: Ixodidae)

作　　者：高志华[1] 杨小龙[1] 刘敬泽[1] 简恒[2] 

机构地区：[1]河北师范大学生命科学学院,石家庄050016 [2]中国农业科学院生物防治研究所,北京100081

出　　处：《昆虫学报》2004年第1期20-24,共5页Acta Entomologica Sinica

基　　金：河北省自然科学基金项目 ( 30 0 1 6 8);河北省人事厅国际合作与交流中心资助项目

摘　　要：用昆虫病原线虫小卷蛾斯氏线虫 (ScBJ)、夜蛾斯氏线虫 (SfOtio)、拟双角斯氏线虫 (ScD43 )、格氏斯氏线虫 (SgNC3 2 )和嗜菌异小杆线虫 (HbE 6 7)对长角血蜱雌蜱进行感染试验 ,所用线虫剂量为 40 0 0IJs dish。结果表明 ,5种线虫均对长角血蜱雌蜱有致死效应。HbE 6 7和ScBJ两种线虫对雌蜱各发育期致病力最强 ,导致雌蜱的累积死亡率和半致死时间分别为饥饿雌蜱 82 5% ,9 0天和 75 0 % ,8 8天 ;吸血雌蜱 90 0 % ,8 0天和 82 5% ,8 0天 ;饱血雌蜱 93 3 % ,7 3天和 86 7% ,7 3天。ScD43对饱血雌蜱有较高的致死效应 ,为 80 0 % ,但半致死时间较长 ,为 11 7天。SfOtio和SgNC3 2对长角血蜱雌蜱的致死效应较低。Infection of entomopathogenic nematodes Steinernema carpocapsae BJ(Sc BJ) ,S. feltiae Otio(Sf Otio),S. ceratophorum D43(Sc D43) and S. gla seri NC32(Sg NC32) (Steinernematidae),and Heterorhabditis bacteriophora E- 6-7 (Hb E-6-7) (Heterorhabditidae) to female Haemaphysalis longicornis Neumann was conducted. The nematodes dosage used was 4 000 IJs/dish. The results showed that the five entomopathogenic nematodes had lethal effects on female H .longicornis. Hb E-6-7 and Sc BJ were the most virulence species to various developmental stages of female ticks: the cumulative mortality and the LT_(50 ) of u nfed female ticks from nematodes infection were 82.5%,9.0 d and 75.0%,8.8 d, respectively; feeding female 90.0%,8.0 d and 82.5%,8.0 d; engorged fema le 93.3%,7.3 d and 86.7%,7.3 d. Sc D43 infection also caused high mortalit y (80.0%) to engorged female ticks, but the LT_(50) was relatively long (11 . 7 d). Lethal effects of Sf Otio and Sg NC32 to this tick were low. Engorged fem ale ticks were more sensitive than unfed and feeding female ticks.

关 键 词：昆虫病原线虫 斯氏线虫 异小杆线虫 长角血蜱 致病力 

分 类 号：Q965[生物学—昆虫学]