甘薯病毒病害SPVD抗性鉴定方法及产量损失估计  

Methodology for identification of disease resistance of sweet potato cultivars to sweet potato virus disease and yield loss estimation

作　　者：王爽[1] 刘顺通 乔奇[1] 张德胜[1] 秦艳红[1] 张振臣[1] 

机构地区：[1]河南省农业科学院植物保护研究所,河南省农作物病虫害防治重点实验室,农业部华北南部作物有害生物综合治理重点实验室,郑州450002 [2]洛阳市农林科学院,洛阳471022

出　　处：《植物保护学报》2014年第2期176-181,共6页Journal of Plant Protection

基　　金：国家甘薯产业技术体系建设项目(CARS-11-B-07);河南省农业科学院科研发展专项

摘　　要：为了建立规范、有效的甘薯病毒病害(sweet potato virus disease,SPVD)抗性鉴定方法,于2011—2012连续两年,利用田间人工嫁接病毒接穗的方法对12个甘薯品种进行抗性鉴定和产量损失测定。结果显示,嫁接接种后,接穗成活率接近100%,12个品种都有不同程度发病,病情指数在51.0~95.2之间;感染SPVD的甘薯植株叶绿素含量降低、蔓长缩短;单株薯块产量损失范围在55.1%~97.8%之间。研究表明,供试的12个甘薯主栽品种感染SPVD后均可引起严重的产量损失,且田间人工嫁接病毒接穗是一个有效的SPVD抗性鉴定方法。An effective method was developed for identification of sweet potato virus disease( SPVD) resistance using artificial grafting virus scions in the field. The resistance of 12 sweet potato varieties was evaluated and the yield losses were estimated from 2011 to 2012. The results showed that survival of the scions was 100%,and 12 sweet potato cultivars were infected with diseases to different degrees after graft inoculation with SPVD and the disease index was between 51. 0 and 95. 2. The leaf chlorophyll content and vine length of sweet potato plants infected with SPVD decreased compared with those of healthy sweet potato plants,and grafting virus scions in the field was an effective method for identification of SPVD resistance. The tuber yield loss was in the range of 55. 1%- 97. 8%. It showed that the 12 sweet potato cultivars tested were susceptible to SPVD.

关 键 词：甘薯病毒病害 抗性鉴定方法 产量损失估计 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]