内生放线菌A-1对苹果果实轮纹病的防效及防御性酶活性的影响  

Control efficiency of endophytic actinomycetes A-1 against apple fruit ring rot and its influence on the activity of defense-related enzymes

作　　者：雍道敬 王彩霞[1] 李桂舫[1] 李保华[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东省植物病虫害综合防控重点实验室,青岛266109

出　　处：《植物保护学报》2014年第3期335-341,共7页Journal of Plant Protection

基　　金：现代农业产业技术体系(CARS-28);公益性行业(农业)科研专项(nyhyzx3-22);山东省科技攻关计划(2010GNC10918);国家自然科学基金(31000891;31272001);山东省"泰山学者"建设工程

摘　　要：为揭示内生放线菌A-1对苹果果实轮纹病的防效及防病机制,采用平板法和喷雾处理刺伤接种法,测定了其对苹果轮纹病菌的抑制作用及果实内防御性酶活性的影响。结果表明:菌株A-1能显著抑制轮纹病菌菌丝生长,104CFU/mL菌悬液的抑制率达90%以上;喷施107CFU/mL A-1菌悬液后,间隔12h以上接种的各处理,3 d和7 d时防效分别为91.79%~95.67%和77.41%~94.00%,均与对照农药苯醚甲环唑相当。喷施107CFU/mL A-1菌悬液后接种或不接种轮纹病菌的处理,果实内过氧化物酶、过氧化氢酶、多酚氧化酶和苯丙氨酸解氨酶活性显著升高,且均高于只接种轮纹病菌的处理;喷施A-1菌悬液后接种病原菌的处理酶活性增加最显著,其峰值是对照的2.30~11.00倍。表明内生放线菌A-1可通过产生拮抗物质、提高寄主防御性酶活性等机制实现对苹果轮纹病的有效防治。In order to reveal the control efficiency and biocontrol mechanism of endophytic actinomycetes strain A-1 against apple fruit ring rot caused by Botryosphaeria dothidea,the inhibitory efficiency of strain A-1 to B. dothidea and its effect on the activities of defense-related enzymes in apple fruits were determined using the agar plate method and spraying strain A-1 combined with wound inoculation. The stain A-1 significantly inhibited the growth of B. dothidea and the inhibitory rate was more than 90%when 104CFU /mL cell culture was used. When applying 107CFU /mL cell culture of strain A-1 for more than 12 h,followed by B. dothidea inoculation,the control efficiency was 91. 79%- 95. 67% or77. 41%- 94. 00% at 3 or 7 d post inoculation,respectively,which was comparable to that of difenoconazole treatment. The activities of peroridase( POD),catalase( CAT),polyphenol oxidase( PPO),and phenylalanine ammonialyase( PAL) were significantly enhanced in apple fruits pre-treated with strain A-1 alone or in combination with B. dothidea inoculation. The enzyme activities increased significantly in fruits treated with 107CFU /mL cell culture of strain A-1,followed by B. dothideainoculation. The activity peaks of PPO,CAT,POD and PAL in treatments were 2. 30 to 11. 00 times higher than those of controls. The endophytic actinomycetes strain A-1 was effective in protecting apple fruit against B. dothidea infection via producing antagonistic substances and increasing the activities of defense-related enzymes.

关 键 词：放线菌A-1 苹果轮纹病菌 防效 防御性酶 生防机制 

分 类 号：S436.611[农业科学—农业昆虫与害虫防治]