G蛋白在杨树气孔运动中的作用  

Roles of G-protein in The Regulation of Stomatal Movement in Poplar

作　　者：周晓阳[1] 张辉[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《科学技术与工程》2004年第1期12-14,27,共4页Science Technology and Engineering

基　　金：国家自然科学基金 ( 3 0 0 0 0 13 0 )资助

摘　　要：群众杨叶片下表皮经过不同浓度的G蛋白激活剂霍乱毒素 (CTX)处理后 ,在扫描电镜下观察了气孔开度的变化 ,并用透射电镜结合X 射线能谱显微分析技术 ,对保卫细胞内的K+ 、Cl- 含量进行了研究。结果表明 :CTX能促进气孔关闭 ,作用强度随CTX浓度的增加而增强。伴随着气孔关闭 ,保卫细胞液泡和细胞质中的K+ 、Cl- 含量都明显下降 ,而细胞壁中的K+ 、Cl- 含量增加。这提示G蛋白可能通过对保卫细胞内K+ 、Cl-Epidermis of P.popularis was treated by CTX. The changes of stomatal aperture were observed by SEM. K +, Cl - contents in vacuole, cytoplasm, cell wall of guard cells were analysed by TEM X-ray microanalysis. The results indicated that CTX promoted stomatal closing. When stomatal closure, K +,Cl - contents decreased obviously in vacuole and cytoplasm, but increased in cell wall of guard cells. It is suggested that G-protein maybe involved in the regulation of stomatal movement.

关 键 词：G蛋白 杨树 气孔运动 霍乱毒素 X-射线能谱显微分析 GtP结合调节蛋白 

分 类 号：Q945.3[生物学—植物学] Q513