血清中异丙酚的HPLC测定  

Determination of propofol in serum by HPLC

作　　者：张慧[1] 沈浩[1] 贾晶莹[1] 曹晖[2] 余琛[1] 

机构地区：[1]上海市徐汇区中心医院中心实验室,上海200031 [2]上海市胸科医院麻醉科,上海200054

出　　处：《中国医药工业杂志》2004年第2期94-95,102,共3页Chinese Journal of Pharmaceuticals

摘　　要：建立了检测血清中异丙酚的反相高效液相色谱-荧光法。血清样品经含内标百里酚的丙酮溶液直接沉淀后,采用KR100-5C18柱,甲醇-0.1%三氟醋酸溶液(80∶20)为流动相,荧光激发波长为276nm,发射波长为307nm。异丙酚在0.05～12.8mg/ml范围内与峰面积比线性关系良好。检测限为150pg,异丙酚和百里酚的提取回收率均大于90%,批内、批间精密度为1.11%～6.88%。A HPLC method with fluorescence detection or determination of propofol in serum was established. Theserum sample was treated with acetone as precipitant and thymol as internal standard. A KR100-5C18 column was used, withthe mobile phase of methanol-0.1%TFA solution (80∶20). The excitation and emission wavelength were 276nm and307nm, respectively. The flow rate was 1.0 ml/min. The calibration curve was linear in the range of 0.05 ～12.8mg/ml. Thedetection limit was 150pg. The extraction recovery was more than 90%. The RSDs of intra- and inter-batch were 1.11%～6.88%.

关 键 词：异丙酚 血药浓度 高效液相色谱-荧光检测 直接沉淀法 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]