加味苓桂术甘汤联合限食对脾虚痰湿型糖尿病脑病大鼠海马组织PI3K/Akt/mTOR通路的影响  

Effects of Modified Linggui Zhugan Decoction Combined with Dietary Restrictions on PI3K/Akt/mTOR Pathway in Hippocampus of Rats with Spleen Deficiency and Phlegm Dampness-induced Diabetic Encephalopathy

作　　者：师林[1] 柯斌[2] 杨玉彬[2] 黄颖娟[2] 秦鉴[2] 张诗军[2] SHI Lin;KE Bin;YANG Yu-bin;HUANG Ying-juan;QIN Jian;ZHANG Shi-jun(Traditional Chinese Medicine Department,Zhu Jiang Hospital of Southern Medical University,Guangzhou 510282,China;Traditional Chinese Medicine Department,First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]南方医科大学珠江医院中医科,广东广州510282 [2]中山大学附属第一医院中医科,广东广州510080

出　　处：《中药材》2018年第5期1186-1190,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81302877);广东省科技计划项目(2014A020212056);广东省中医药局项目(20141057;20161056)

摘　　要：目的:研究加味苓桂术甘汤联合限食对脾虚痰湿型糖尿病脑病(DE)大鼠海马组织PI3K/Akt/mTOR通路的影响。方法:建立脾虚痰湿型糖尿病脑病大鼠模型,将大鼠随机分为正常对照组、DE组、脾虚痰湿DE组、限食组、加味苓桂术甘汤组、加味苓桂术甘汤联合限食组和二甲双胍组,药物干预4 w。给药后测量体质量、空腹血糖、血脂;Morris水迷宫检测大鼠学习记忆能力;Western blot检测各组海马组织PI3K、Akt、mTOR蛋白表达。结果:加味苓桂术甘汤联合限食可显著降低脾虚痰湿型糖尿病脑病大鼠体质量、空腹血糖、血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平(P<0.05或P<0.01);改善Morris水迷宫实验成绩(P<0.05);升高海马组织PI3K、Akt蛋白表达(P<0.01),降低mTOR蛋白表达(P<0.01)。结论:加味苓桂术甘汤联合限食能改善脾虚痰湿型糖尿病脑病大鼠糖脂代谢,增强学习记忆能力,其机制可能与调节PI3K/Akt/mTOR通路有关。Objective:To study the effect of modified Linggui Zhugan decoction combined with dietary restrictions on PI3 K/Akt/mTOR pathway in hippocampus of rats with spleen deficiency and phlegm dampness-induced diabetic encephalopathy(DE).Methods:The rat model of diabetic nephropathy with spleen deficiency and phlegm dampness was established.The rats were randomly divided into normal group,DE group,DE with spleen deficiency and phlegm dampness group,limited diet group,modified Linggui Zhugan decoction group,combination of modified Linggui Zhugan decoction and limited diet group and metformin group.Drugs were intervened for 4 w.After administration,body mass,fasting blood glucose and serum lipids were measured.Morris water maze was used to detect the learning and memorizing ability of rats.Western blot was used to detect the protein expression of PI3 K,Akt and mTOR in each group.Results:Modified Lingguizhugan decoction combined with limited diet could obviously reduce the levels of body weight,fasting blood glucose,serum of tal cholesterol,triglyceride and low density lipoprotein cholesterol(P<0.05 or P<0.01),and improve morris water maze test scores(P<0.05),increase PI3 K and Akt protein expressions in hippocampus(P<0.01),and decrease the expression of mTOR protein(P<0.01)in rats with spleen deficiency and phlegm dampness induced diabetic encephalopathy.Conclusion:Modified Linggui Zhugan decoction combined with limited diet can improve glucose and lipid metabolism and the abilities of learning and memorizing in rats with spleen deficiency and phlegm dampness induced diabetic encephalopathy.The mechanism may be associated with PI3 K/Akt/mTOR pathway.

关 键 词：糖尿病脑病 热量限制 肥胖 脾虚痰湿证 中医药疗法 

分 类 号：R285.5[医药卫生—中药学]