心灵丸对ApoE-/-小鼠动脉粥样硬化斑块及斑块内血管新生的影响  

Effects of Xinlingwan on Atherosclerosis and Intraplaque Angiogenesis of ApoE-/- Mice

作　　者：梁万洪 黎智文 黄瑞莉[3] 陈爽 吴粤湘 黄锦恺 洪永敦[3] LIANG Wanhong;LI Zhiwen;HUANG Ruili;CHEN Shuang;WU Yuexiang;HUANG Jinkai;HONGYongdun(Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;ShenzhenTraditional Chinese Medicine Hospital of Baoan District,Shenzhen 518000 Guangdong,China;The FirstAffiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China;Guangzhou Hospital of Integrative Chinese and Western Medicine,Guangzhou 510800 Guangdong,China)

机构地区：[1]广州中医药大学,广东广州510405 [2]深圳市宝安区中医院,广东深圳518000 [3]广州中医药大学第一附属医院,广东广州510405 [4]广州市中西医结合医院,广东广州510800

出　　处：《中药新药与临床药理》2019年第9期1088-1092,共5页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的探讨心灵丸对ApoE-/-小鼠动脉粥样硬化(AS)易损斑块及斑块内血管新生的作用。方法以高脂饲料喂养雄性ApoE-/-小鼠13周,复制AS小鼠模型。将AS模型小鼠随机分成模型组(给予等量蒸馏水)、辛伐他汀组(2.6 mg·kg-1)、心灵丸组(15.6 mg·kg-1),每组15只,灌胃给药,每天1次,连续13周。测定小鼠动脉斑块内成分:细胞外脂质成分、泡沫细胞、平滑肌细胞、胶原纤维,计算斑块易损指数;采用CD34法测定斑块内新生血管的密度。结果与模型组比较,辛伐他汀组及心灵丸组的斑块易损指数、斑块内新生血管密度均有明显降低(P<0.05);心灵丸组与辛伐他汀组在降低斑块易损指数、斑块内新生血管密度方面的差异无统计学意义(P>0.05)。结论心灵丸可通过减少AS斑块内泡沫细胞,增加斑块内平滑肌及胶原成分,降低斑块内新生血管的表达以稳定AS斑块。Objective To investigate the effects of Xinlingwan on the atherosclerotic vulnerable plaque and intraplaque angiogenesis of ApoE-/-mice.Methods A total of 60 8-week male ApoE-/-mice were fed with high-fat diet for 13 weeks to induce atherosclerosis(AS).And then the model mice were randomly divided into model group,simvastatin group(2.6 mg·kg-1)and Xinlingwan group(15.6 mg·kg-1).Distilled water,simvastatin and Xinlingwan were given to the corresponding groups following the experimental design for another successive 13 weeks.The components of arterial plaque of each group,including extracellular lipid composition(ELC),foam cells(FC),smooth muscle cells(SMC),collagenous fibers(CF),were measured and counted.The atherosclerotic plaque vulnerability index and new vessel density were calculated.Results The vulnerability index and intraplaque microvessel density of the simvastatin group and the Xinlingwan group mice were lower than those of model group(P<0.05).There was no difference between the simvastatin group and the Xinlingwan group in reducing the vulnerability index and neovascular density in the plaque(P>0.05).Conclusion It showed that Xinlingwan could stabilize AS plaque by decreasing intraplaque foam cells and microvessel density,and increasing SMC and CF in intraplaque.

关 键 词：心灵丸 动脉粥样硬化 易损斑块 易损指数 血管新生 

分 类 号：R285.5[医药卫生—中药学]