丹参酮ⅡA抑制血管紧张素Ⅱ诱导的大鼠心肌细胞肥大  

TanshinoneⅡA prevents angiotensinⅡ-induced hypertrophy of cardiomyocytes from rat

作　　者：龚丽娅[1] 郑智[1] 熊玮[1] 孙联平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北武汉430030

出　　处：《华西药学杂志》2004年第1期24-27,共4页West China Journal of Pharmaceutical Sciences

基　　金：湖北省科技攻关课题 ( 2 0 0 2AA3 0 1C2 9)

摘　　要：目的　在原代培养的新生大鼠心肌细胞上 ,观察丹参酮ⅡA(TSN)对血管紧张素Ⅱ (AngⅡ )诱导的心肌细胞肥大的影响。方法　采用3 H -亮氨酸掺入法测定心肌细胞蛋白质合成速率 ,作为心肌细胞肥大的指标 ;用逆转录聚合酶链式反应检测心肌细胞原癌基因c -fosmRNA的表达。结果　TSN能抑制AngⅡ诱导的大鼠心肌细胞蛋白质合成的增加 (P <0 .0 5 )及心肌细胞原癌基因c -fosmRNA表达的增强 (P <0 .0 5 ) ,其效果与AngⅡ受体阻滞剂缬沙坦相似。结论　TSN可以抑制AngⅡ诱导的大鼠心肌细胞肥大 ,这与其抑制了原癌基因c-fos的表达有关。OBJECTIVE To investigate the effect of TanshinoneⅡA(TSN) on the hypertrophy induced by angiotensinⅡ(AngⅡ)in the primary culture of neonatal rat cardiomyocytes.METHODS As an index of cardiomyocyte hypertrophy,protein synthesis rate was measured by 3H-Leucine incorporation.The proto-oncogene c-fos mRNA expression was assessed using reverse transcription polymerase chain reaction(RT-PCR).RESULTS Protein synthesis rate and proto-oncogene c-fos mRNA expression of cardiomyocytes increased significantly after AngⅡtreatment,and TSN inhibited these effects of AngⅡ.CONCLUSION TSN can prevent the hypertrophy of cardiomyocytes induced by AngⅡ,which may relate to the decreased expression of proto-oncogene c-fos mRNA by TSN.

关 键 词：丹参酮ⅡA 抑制 血管紧张素Ⅱ 诱导 心肌细胞肥大 

分 类 号：R285.5[医药卫生—中药学]