白血病细胞的^(31)P核磁共振分析及用于无损伤游离镁离子测定  

Determination of Intracellular Metabolite and Cytosolic Free Magnesium in HL-60 Cells by Using ^(31)P-Nuclear Magnetic Resonance

作　　者：黄荣清[1] 骆传环[1] 杜泽涵[2] 肖炳坤[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《分析化学》2004年第2期129-133,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (No .C0 3 0 5 0 2 5 0 ;2 990 5 0 0 5 );北京市自然科学基金 (No .70 0 2 0 2 9)资助项目

摘　　要：建立了无损伤性3 1P NMR研究细胞内代谢物的实验方法 ,并对人早幼粒白血病细胞HL 6 0的3 1P NMR谱中含磷小分子代谢物的谱峰进行了分析 ;通过测量HL 6 0的3 1P NMR谱中ATP的α磷和 β磷的化学位移差值 ,得出HL 6 0细胞内Mg2 + 与ATP结合的复合物MgATP和整个ATP量的比值 ,计算得到HL 6 0细胞内游离Mg2 + 浓度为 0 .2 6 4mmol/L。与其它分析方法相比 ,3 1P NMR测定细胞内游离Mg2 +P nuclear magnetic resonance (NMR) has been established to nondestructively measure intracellular metabolite in living cells. Stable 31P-NMR spectra of living cells were obtained and intracellular metabolite in human promyelocyte leukemia cell line HL-60 were analyzied. Based upon a measurement of chemical shift differences between the α-phosphoryl and β-phosphoryl resonances in the 31P-NMR spectrum of intracellular adenosine triphosphate (ATP) in human promyelocyte leukemia cell line HL-60, the fraction of total ATP that is complexed to Mg 2+ was estimated. Cytosolic free magnesium ion concentration in HL-60-4 cells was measured nondestractively to be 264.4 mmol/L.

关 键 词：白血病细胞 无损伤性 磷31核磁共振 游离镁离子 含量测定 

分 类 号：O657.2[理学—分析化学]