压力对加压电色谱分离选择性影响的研究  

Character of Separation Selectivity in Pressurized Capillary Electrochromatography

作　　者：曹枫[1] 张维冰[2] 阎超[2] 张玉奎[2] 

机构地区：[1]安徽工业大学,马鞍山243011 [2]中国科学院大连化学物理研究所,国家色谱研究分析中心大连116011

出　　处：《分析化学》2004年第2期143-147,共5页Chinese Journal of Analytical Chemistry

基　　金：国家重点基础研究发展规划 ( 973 )项目 (No .0 0 1CB5 10 2 0 2 );国家自然科学基金 (No .2 0 10 5 0 0 6)

摘　　要：基于弛豫理论的基本原理 ,通过对溶质在加压电色谱中输运过程的研究 ,得到了溶质多种形态同时存在情况下反映其色谱行为与电泳行为对迁移时间影响的理论表达式。进一步研究了中性溶质与中性溶质、中性溶质与带电溶质以及带电溶质与带电溶质的分离选择性与压力、操作电压等的关系 ,结果表明 :对于中性溶质的分离 ,尽管可以通过压力的改变调节峰间距和分离时间 ,但不会对出峰次序产生影响。对于中性溶质与带电溶质的分离以及带电溶质与带电溶质的分离 。Based on the method of relaxation theory, an expression, which described the relationship between moving time of solute and electrophoretic flow, electroosmotic flow (EOF) and pressurized flow, was theoretically derived. The separation selectivity of neutral and neutral species, neutral and charged species, charged and charged species was further discussed. The results reveal that the separation selectivity of neutral and charged species, charged and charged species can be conveniently adjusted by voltage or pressure, but the peak order of separation of neutral and neutral species would not be effected.

关 键 词：加压电色谱 弛豫理论 分离选择性 压力 溶质 

分 类 号：O657.7[理学—分析化学]