Urocortin, the neuropeptide, inhibits the viability of ECV304 cells and rat vascular smooth muscle cells  

神经肽Urocortin抑制ECV304细胞和鼠平滑肌细胞的增值(英文)

作　　者：陈洁[1] 汪红仪[1] 陶金[1] 徐华娥[1] 杨荣[2] 李胜男[1] 

机构地区：[1]南京医科大学药理学系,南京210029 [2]南京医科大学第一附属医院心内科

出　　处：《Journal of Nanjing Medical University》2004年第1期1-3,共3页南京医科大学学报（英文版）

基　　金：SupportedbygrantfromNationalScientificFundofJiangsuProvinceEducationCommittee ( 0 2KJB3 10 0 0 6) ,MinistryofEducationReturningOverseasScholarScienceStudyFoundationandScientificDevelopmentalFundofNanjingMedicalUniversity(NY0 3 0 63 )

摘　　要：Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' thoracic aorta. We studied the effect of Ucn on the viability of ECV304 cells and VSMC by using a tetrazolium (MTT) assay.Results: Ucn (10 -7 mol/L) inhibited the viability of ECV304 cells and VSMC. Inhibition rates are 13% and 15%, respectively(P<0.05, compared with Control). This inhibition was not dependent on the affecting time and was not affected by the addition of ATP-sensitive potassium channel (KATP channel) blocker, glybenclamide (Gly, 10 mol/L). Conclusion: Ucn inhibits the viability of ECV304 and VSMC. Our results suggest that Ucn may be a new vasoactive agent and may have a beneficial effect in the process of vascular remodeling (VR).目的 :本实验目的在于研究Urocortin(Ucn)对内皮细胞 (一种取自人脐静脉内皮细胞HUVECECV30 4 )以及大鼠动脉平滑肌细胞 (Vascularsmoothmusclecells ,VSMC)增值的影响 ,从而探讨其在血管重构 (VascularRe modeling ,YR)中的作用。方法 :通过四唑盐比色法研究Ucn对内皮细胞以及鼠平滑肌细胞增值的影响。结果 :Ucn(10 -7mol/L)抑制ECV30 4和VSMC细胞的增值。这种抑制作用不依赖于作用时间也不受ATP敏感的钾离子通道阻滞剂glybenclaimide(Gly ,10 μmol/L)的影响。 结论 :提示Ucn不依赖钾离子通道的作用来控制血管重构。可能对高血压的脑或其他器官的并发症有防治作用 。

关 键 词：UROCORTIN ECV304 vascular smooth muscle cells MTT assay ATP-sensitive potassium channels 

分 类 号：R965[医药卫生—药理学]