甘蔗斑茅的杂交利用及其杂种后代鉴定系列研究(二):甘蔗斑茅远缘真实杂种的分子鉴定  

Utilization and Characterisation of the Genuine Intergeneric Hybrids from the Cross of Saccharum and E.arundinaceum (2):Molecular Identification of Genuine Hybrids from the Cross of Saccharum and E.arundinaceum

作　　者：郑雪芳[1] 张木清[2] 李奇伟[2] 劳方业[2] 邓祖湖[1] 陈如凯[1] 杨业后[2] 

机构地区：[1]福建农林大学农业部甘蔗遗传育种重点开放实验室,福州350002 [2]广州甘蔗糖业研究所,广州510316

出　　处：《分子植物育种》2004年第1期35-42,共8页Molecular Plant Breeding

基　　金：国家“863”计划课题糖料新品种选育(2001AA241191);国家自然科学基金(30170590)资助。

摘　　要：根据斑茅ITS区序列设计编号为EF1/ER1和编号为EF2 /ER2 的两对引物 ,经在甘蔗及其近缘属植物间ITS PCR扩增 ,证明这两对引物是斑茅特异引物 ,其中引物EF1/ER1在斑茅多态性研究中扩增出 5种带型 ,而引物EF2 /ER2 扩增出 3种带型 ;同时 ,利用这两对引物对甘蔗 (拔地拉 )与斑茅杂交的 9个F1,甘蔗斑茅真实杂种 (崖城 96 - 6 6、 96 - 46 )与CP84 - 1198的 36个BC1以及崖城 95 - 41与内江 5 7- 416的 12个BC1,15个曾被认为具有斑茅血缘的品系进行分子鉴定 ,结果表明 ,两对引物的鉴定结果基本一致 ,其中对斑茅F1的分子鉴定结果与前人 (沈万宽 )的同工酶鉴定结果相一致 ,对 15个曾被认为具有斑茅血缘的品系进行鉴定结果显示大部分品系为假杂种。Based on the sequence of internal transcriped spacer(ITS) region of E.arundinaceum, two primers were designed and named as EF 1/ER 1 and EF 2/ER 2. They were proved as the specific primers for E.arundinaceum by comparing Saccharum with its related generas. Results showed that primer EF 1/ER 1 produced five kinds of band, primer EF 2/ER 2 produced 3 kinds of band from 30 clones of E.arundinaceum collected in Yacheng of the Southernmost of China. While using these two primers to identificated nine F 1 from the cross of Saccharum and E.arundinaceum、thirty-six BC 1 from the cross of hybrids of E.arundinaceum (YC96-66,YC96-46) and CP84-1198、twelve BC 1 from the cross of YC95-41 and Neijiang57-416 and fifteen clones, the genuine hybrids were identified. The results of moleculer identification for F 1 hybrids of E.arundinaceum is accorded with isozyme identification that was reviewed by Shen W.K. However, most of fifteen clones which had been considered as the genuine hybrids from the cross of Saccharum and E.arundinaceum, were not genuine hybrids in this research.

关 键 词：甘蔗 斑茅 杂交利用 杂种鉴定 分子鉴定 ITS分子标记 

分 类 号：S566.1[农业科学—作物学]