利用SSR标记辅助选择杂交组合亲本  

Assisted Selecting Parents of Hybrid Combination Based on SSR Polymorphism

作　　者：黄育民[1] 王侯聪[1] 马磊[1] 

机构地区：[1]厦门大学生命科学院,厦门361005

出　　处：《分子植物育种》2004年第1期43-47,共5页Molecular Plant Breeding

基　　金：国家863计划项目(2002 AA211091)资助

摘　　要：本研究利用 6 0对SSR引物对籼稻品种佳辐占与 6个候选亲本品种进行相互之间的多态性分析。结果表明 :佳辐占与明恢 6 3之间的多态性程度最高 ,达到 2 2 7% ;其次是与明恢 86之间的多态性程度 ,达 19 6 % ,而与其他品种的多态性程度较低。多态性程度高的品种间的杂交组合 ,其杂种优势明显高于多态性程度低的品种间的组合。初步表明 ,根据SSR多态性程度的高低选配亲本 ,其配制杂交组合可以获得较高的杂种优势。In this research, the authors attempt to select the parents for hybrid combination by using SSR polymorphism. Indica rices, Jiafuzhan and other six candidate parents, were employed to be detected and analyzed DNA polymorphism by using sixty pairs of SSR primers. It showed that the degree of polymorphism between Jianfuzhan and Minghui 63 was highest, it reaches 22.7%, and of that between Jianfuzhan and Minghui 86 ranks the second, it reaches 19.6%. it is lower of the degree of polymorphism between Jiafuzhan and other 4 varieties. The heterosis of the combination was according to the degree of polymorphism between parents, the higher polymorphic degree between parents the higher heterosis the combinations. The results showed that it was feasible to select the parents of hybrid combinations based on SSR polymorphism for evaluating the heterosis of the hybrid combinations.

关 键 词：籼稻 杂交组合 亲本选择 SSR标记辅助选择 遗传多态性 杂种优势 

分 类 号：S511.21[农业科学—作物学]