HPLC法测定不同采收期杭白菊中木犀草素及其苷的含量  

Determination of luteolin and luteolin-7-β-D-glucoside in Chrysanthemum morfolium Ramat. from different collection time by RP-HPLC

作　　者：胡碧波[1] 蒋惠娣[1] 杨俊[1] 曾苏[1] 

机构地区：[1]浙江大学药学院药物分析与药物代谢研究室,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2004年第1期29-32,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省中医药局资助;浙江省科技厅重点资助项目

摘　　要：目的 :测定杭白菊中木犀草素和木犀草素 - 7- β- D-葡萄糖苷的含量 ,考察它们在采摘期内的含量变化。方法 :反相高效液相色谱法测定 2 0 0 1、2 0 0 2年度不同采摘期杭白菊中两者的含量。结果 :杭白菊中木犀草素含量大大低于木犀草素 - 7- β- D-葡萄糖苷含量 ;木犀草素含量在采摘期中无显著变化 ,而木犀草素 - 7- β- D-葡萄糖苷含量在采摘初期变化不明显 ,而在采摘后期则呈下降趋势。结论 :杭白菊中木犀草素 - 7- β- D-葡萄糖苷含量更能反映杭白菊品质。Objective: To observe the content variation of luteolin and luteolin-7-β-D-glucoside in Chrysanthemum morifolium Ramat. (CMR) from different collection time. Methods: RP-HPLC was used to analyze these two components in CMR collected in 2001 and 2002. Results: The content of luteolin was significantly lower than that of luteolin-7-β-D-glucoside. Furthermore, the former showed no marked changes during collection,while the latter did not varied markedly in early collection but decreased significantly in later collection. Conclusion: The content of luteolin-7-β-D-glucoside reflects the quality of Chrysanthemum morifolium Ramat. more viably than that of luteolin.

关 键 词：杭白菊 分析 黄酮类 色谱法 高效液相 

分 类 号：R284.2[医药卫生—中药学]