无溶剂体系酪氨酸酶催化对位苯酚类化合物与芳香胺的反应研究  

Research on Solvent-free Reaction of 4-Substituded Phenols with 2-Methoxyaniline Catalyzed by Tyrosinase

作　　者：徐迪[1] 黄成红[1] 郭新东[1] 马林[1] 古练权[1] 

机构地区：[1]中山大学化学与化工学院,广东广州510275

出　　处：《分子催化》2004年第1期10-14,共5页Journal of Molecular Catalysis(China)

基　　金：国家自然科学基金(20074042)资助项目.

摘　　要：以对苄氧基苯酚,对苄基苯酚和对苯氧基苯酚作为底物,研究在无溶剂体系下,酪氨酸酶分别催化它们与邻氨基苯甲醚的反应.考察了在无溶剂体系下,以底物200μmol为当量,酶催化该类反应的最适水含量为30～35μL,加酶量为35～40mg,反应时间为24h.以此为反应条件酶促催化合成了2和3b,3a,产率30～50%,并讨论了反应机制.In this article, reaction of 4-substituded phenols(4-hydroxydiphenylmethane, 4-phenoxyonenol, 4-benzyloxyphenol) with 2-methoxyaniline catalyzed by tyrosinase in solvent-free system was intensively studied. The effects of water content, reaction time, amount of enzyme were investigated, and the reaction products2, 3a and 3b were obtained with the yield of 35~50%. The mechanism catalyzed by tyrosinase in solvent-free systems was explained.

关 键 词：无溶剂 酪氨酸酶 氧化-迈克尔加成 4-苯氧基苯酚 4-苄氧基苯酚 4-苄基苯酚 

分 类 号：O643.32[理学—物理化学]