哺乳类细胞位点突变试验的改进及应用  

THE MODIFIED MAMMALIAN CELLS LOCUS ASSAY AND ITS APPLICATION

作　　者：叶舜华[1] 祁萍萍[1] 杨小平 庞明 吴国强 

机构地区：[1]上海医科大学公共卫生学院

出　　处：《现代预防医学》1992年第1期1-4,共4页Modern Preventive Medicine

摘　　要：用人表皮细胞的HGPRT基因位点测试,从放射自显影计数法,改为液体闪烁法测定。两法比较。结果呈正相关,r=0.98。用改进法检验了一些环境污染物对V_(79)细胞的诱变性。结果提示卷烟焦油具有直接的诱变作用,厨房油烟冷凝物为间接诱变物,大气飘尘和铅的诱变性尚难肯定。A modified mammalian cells locus mutation assay was used to demonstrate the HGPRT mutation in human keratinocytes exposed to lead in vitro. Liquidscintillation spectrometry and autoradiographic methods were studied. The relationship between the two methods showed a positive correlation (r=0.98). The results showed that tobacco tar possessed a direct acting mutagenicity to mammalian cells. Cooking oil fume condensates were indirectly and slightly mutagenic. The mutagenicity of lead and atmospheric suspended particulates are not yet determined.

关 键 词：HGPRT 基因位点试验 

分 类 号：R994.6[医药卫生—毒理学]