气相色谱—质谱联机分离鉴定叶枯灵的代谢物  

ISOLATION AND IDENTIFICATION OF METABOLITES OF YEKULING BY GASCHROMATOGRAPHIC-MASS SPECTROMETRY

作　　者：王宇[1] 李正化[1] 高宁[2] 

机构地区：[1]华西医科大学药学院新药设计研究所 [2]第三军医大学卫防系环境卫生学教研究室

出　　处：《现代预防医学》1992年第4期193-194,共2页Modern Preventive Medicine

基　　金："七.五"国家科技攻关项目

摘　　要：经口灌胃给大鼠叶枯灵35.8mg/kg,收集24小时尿,采用气相色谱—质谱(GC—MS)联机分离和鉴定了尿中的主要代谢物。据此推断,叶枯灵进入体内迅速水解,继而在氮原子上乙酰化,并用化学合成品对照确证,该法灵敏度高,分离效果好。Rats were given. 35.8mg/kg of yekuling orally and urine was collected 24 hours later.The main metabolites in the urine were isolated and identified by GC-MS. It may be considered that Yekuling was readily hydrolyzed to Benzoic acid and 1.3-dithiolan-2-ylidene-hydrazine in rat, after which 1.3-dithiolan-2-ylidenehydrazine was acetylated at the nitrogenatom. Then the presence of these metabolites was further confirmed by chemical synthesiS. The sensitivity and isolation are good.

关 键 词：叶枯灵 代谢物 GC-MS 

分 类 号：R139.3[医药卫生—劳动卫生]