BTH诱导水稻对稻瘟病系统获得抗性的mRNA差别显示分析  

mRNA Differential Display of Systemic Acquired Resistance Induced by BTH in Rice against Blast Caused by Magnaporthe grisea

作　　者：饶志明[1] 董海涛[2] 庄杰云[3] 柴荣耀[4] 李德葆[2] 郑康乐[3] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036 [2]浙江大学农业部植物病理学重点实验室,浙江杭州310029 [3]中国水稻研究所国家水稻改良中心,浙江杭州310006 [4]浙江省农业科学院植物保护研究所,浙江杭州310021

出　　处：《无锡轻工大学学报（食品与生物技术）》2004年第1期25-29,35,共6页Journal of Wuxi University of Light Industry

基　　金：国家自然科学基金项目(30070400);浙江省自然科学基金项目(300006)资助课题.

摘　　要：运用mRNA差别显示技术对水稻感病品系G71受苯并噻二唑(Benzothiadiazole,BTH)诱导3d的应答反应进行了分析,从抗病及其相关基因保守结构域设计的10个引物组合的反应中获得了11个受BTH诱导的cDNA差异片段.反向Northern鉴定其中3个为阳性片段.Northern证实G71NBS14BI2的表达确实受BTH和稻瘟病菌的诱导.RFLP分析将G71NBS14BI2定位于水稻的第11条染色体上,且该片段座落于控制水稻穗瘟部分抗性的QTL区间.The expression profiles a line G71 susceptible to isolate 92-183in response to Benzothiadiazole(BTH) were investigated using mRNA differentially display (DD). The leaves were sprayed with BTH for 3 daysat 4-leaf stage.A total of 11 differentially expressed cDNA fragments were obtained with 10 combinations of primersdesigned according to the conserved domains of resistance or resistance related genes. Reverse Northern identified that 3cDNA fragments were up- regulated by spraying of BTH. Northern blot confirmed the expression of G71NBS14BI2 was induced by BTH and Magnaporthe grisea. G71NBS14BI2 was mapped on chromosome 11 by RFLP analysis and located in the interval where QTL controlling partial resistance to neck blast was detected.

关 键 词：BTH诱导 水稻 稻瘟病 MRNA差别显示 基因定位 系统获得抗性 

分 类 号：S511[农业科学—作物学]