大豆5个花叶病毒株系抗性基因的定位  

Mapping of Five Genes Resistant to SMV Strains in Soybean

作　　者：王永军[1,2] 东方阳[2] 王修强[1] 杨雅麟[1] 喻德跃[1] 盖钧镒[1] 吴晓雷[2] 贺超英[2] 张劲松[2] 陈受宜[2] 

机构地区：[1]南京农业大学大豆研究所,国家大豆改良中心,作物遗传与种质创新国家重点实验室,南京210095 [2]中国科学院遗传与发育生物学研究所植物生物技术开放实验室,北京100101

出　　处：《Acta Genetica Sinica》2004年第1期87-90,共4页

基　　金：国家 8 63项目 (编号 :10 1 0 2 0 2 0 3 );国家重点基础研究项目 (编号 :G19980 10 2 0 0 )资助南京农业大学大豆研究所与中国科学院遗传与发育生物学研究所合作项目~~

摘　　要：以科丰 1号×南农 1138 2为亲本构建的RIL群体NJRIKY为材料 ,对群体进行了 5个SMV株系 (Sa、Sc 8、Sc 9、N1 、N3)的抗病性鉴定。结果表明 :大豆对 5个SMV株系的抗性均受一对显性基因控制。用Mapmaker 3 0进行连锁分析 ,发现Rsa与Rn1、Rn3和Rsc9均连锁 ,距离分别为 2 1 4cM、2 3 5cM和 35 3cM ;Rsc8只和Rn1连锁 ,距离为 35 8cM ;Rn1和Rn3之间的遗传距离最近 ,为 10 2cM。多点分析结果表明 :5个抗病基因的排列顺序和遗传距离为Rsc8 35 8cM Rn1 10 3cM Rn3 2 1 5cM Rsa 35 8cM Rsc9。根据RFLP、SSR标记分析结果构建了一套大豆遗传图谱 ,该图谱包含 2 2个连锁群、2 5 6个标记 ,总遗传距离为 30 5 0 9cM。将 5个抗病基因定位于N8 D1b +W连锁群 ,有 3个RFLP标记和Rn1、Rn3都连锁 ,分别为A6 91T、K4 77I、LC5T。它们与Rn1、Rn3的距离分别为 15 0 4cM、17 82cM、15 37cM和 16 14cM、17 82cM、16 5 8cM。Soybean mosaic virus (SMV) is one of the most prevalent pathogens that impact the soybean world widely.Previous reports showed that most of the resistances were controlled by one pair of dominant genes.In this study,a soybean RIL population NJRIKY derived from Kefeng 1× Nannong 1138-2 was used to study the inheritance of resistance to five SMV strains (Sa,Sc-8,Sc-9,N_1,and N_3) and mapping of resistant genes.Kefeng 1 is resistant to all five SMV strains while Nannong 1138-2 is susceptible to all five SMV strains.The parents and RIL populations were planted in green house and five SMV strains were inoculated on different populations.The results showed that each ratio of the number of resistant families to that of susceptible families was consistent with 1∶1 for the five strains.This indicated that the resistance to each of the five strains was controlled by one dominant gene,respectively.RFLP and SSR markers were used to analyze the RIL population,Mapmaker/Exp 3.0b was used to study the linkage between markers and the resistant genes.Through linkage analysis,Rsa was found linked Rn1,Rn3 and Rsc9 with 21.4 cM,23.5 cM and 35.3 cM,Rsc8 was found to be linked only Rn1 with 35.8 cM.Multi locus analysis showed that the order and intervals of the five resistance genes were Rsc8-35.8 cM-Rn1-10.3 cM-Rn3-21.5 cM-Rsa-35.8 cM-Rsc9.According to the result of RFLP and SSR analysis,a genetic map was constructed which consisted of 256 markers that covered 3050.9 cM and converged into 22 linkage groups.The five resistance genes were mapped on the linkage group N8-D1b+W.The RFLP markers A691T,K477I,LC5T were found linked to the resistant genes Rn1 and Rn3 with distances of 15.04 cM?17.82 cM?15.37 cM,16.14 cM?17.82 cM?16.58 cM.

关 键 词：大豆花叶病毒 抗病基因 遗传图谱 基因定位 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]