9-顺-维甲酸对肺癌细胞周期及周期因子表达的影响  

Effects of 9-cis-retinoic acid on cell cycle and expression of cyclin D1 and cdk4 in lung cancer cells

作　　者：任桂杰[1] 刘志方[1] 胡国强[2] 胡晓燕[1] 田克立[1] 于雪艳[1] 

机构地区：[1]山东大学医学院生化与分子生物学研究所 [2]山东大学齐鲁医院,山东济南250012

出　　处：《药学学报》2004年第2期97-100,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(39770 730 )

摘　　要：目的　探讨 9 顺 维甲酸对肺癌细胞周期及周期因子cyclinD1和cdk4表达的影响。方法　用 9 顺 维甲酸处理细胞 2 4h后 ,在观察细胞生长、流式细胞仪分析细胞周期的基础上 ,用RT PCR方法分析细胞周期因子cyclinD1和cdk4表达的变化。结果　 9 顺 维甲酸作用于肺癌细胞 2 4h以后 ,细胞增殖减慢 ,G0 G1期细胞明显增多 ,S期细胞减少 ;PG ,SPC A1和L78细胞的cyclinD1表达下降显著 ;PG ,A549和L78细胞的cdk4表达下降显著。结论　 9 顺Aim To study the effects of 9-cis-retinoic acid (9-cis-RA) on cell cycle and expression of cyclin D1 and cdk4 in lung cancer cells. Methods 9-cis-RA (1×10 -6 mol·L -1 ) was used to treat lung cancer cells for 24 h; Flow cytometry (FCM) was used to detect the percent of G 0/G 1 phase and S phase cells of three groups including blank control, DMSO control a nd 9-cis-RA groups; RT-PCR was used to analyze the expression changes of cyclin D1 and cdk4 before and after treatment with 9-cis-RA in lung cancer cells. Results The percent of G 0/G 1 phase cells of 9-cis-RA groups was significantly higher than that of the control groups (P<0 01 or P<0 05) and the perc ent of S phase cells of 9-cis-RA groups was lower than that of the control groups (P<0 01 or P<0 05); the expression of cyclin D1 of PG, SPC-A1 and L78 cells was decreased (P<0 01) and the expression of cdk4 of PG, A54 9 and L78 cells was also decreased (P<0 01) after treatment with 9-cis -RA. Conclusion Most of the proliferation and the expression of cyclin D1 and cdk4 of PG, A549, SPC-A1 and L78 were inhibited by 9-cis-RA.

关 键 词：9-顺-维甲酸 肺癌 细胞周期 cvclinDl CDK4 药物治疗 

分 类 号：R979.1[医药卫生—药品] R734.2[医药卫生—药学]