应用光学微操作技术分选单条水稻染色体  

OPTICAL TECHNIC OF ISOLATION A SINGLE CHROMOSOME

作　　者：王浩威[1] 刘晓辉[2] 李银妹[1] 韩斌[2] 楼立人[1] 王康俊[1] 

机构地区：[1]中国科技大学选键化学重点实验室 [2]中国科学院上海生命科学研究院国家基因研究中心,上海200233

出　　处：《生物物理学报》2004年第1期50-56,共7页Acta Biophysica Sinica

基　　金：上海市科委科学基金攻关项目;国家自然科学基金重大项目(19890380);国家科委基础研究项目

摘　　要：报道了一种基于光镊技术的实用的单条染色体分选技术。具体介绍了用光镊与光刀结合,并辅以微吸管分选水稻单条染色体的过程。通过该方法得到的水稻单条染色体样品经过分子克隆,制备出了染色体特异的DNA片段并用于水稻基因组测序工作。还将光学微操作技术与现有的几种分选单条染色体的方法(如玻璃微针挑取、激光弹射以及流式细胞仪等)进行了比较。与这些方法相比,光学微操作方法具有液相环境中分离、操作简易、对染色体损伤小、选择性高、无污染等优点。A new method based on the optical tweezers techno lo gy was reported for isolation of single chromosome. In this paper, we introduced the isolation of single rice chromosome with optical tweez ers and optical scaple. A rice cell suspended in the liquid was firs t crushed by a short laser pulse. Then a released chromosome from th e cell can be manipulated by optical tweezers and pulled away from o ther cells and oddments without any direct contact. Finally it was ex tracted individually into a glass capillary nearby. After molecular clo ning, we can obtain some specific DNA segments from the individual ch romosome. All these segments can be used for rice genomic sequencing. Different methods, by which we can extract single chromosome, were co mpared. The advantages of optical micromanipulation method were summariz ed.

关 键 词：光镊 光刀 染色体 基因组 光学微操作技术 水稻 

分 类 号：Q343.2[生物学—遗传学] S511[农业科学—作物学]