蛋白质电泳在豌豆品种鉴定中的应用  

Application of Proteins Gel Electrophoresis in The Identification of Pea Cultivars

作　　者：孙雁[1] 朱有勇[1] 朱永平[2] 杨琼珍 

机构地区：[1]云南农业大学云南省植物病理重点实验室,昆明650201 [2]云南农业大学农学与生物技术学院,昆明650201 [3]云南省个旧市种子公司,个旧661400

出　　处：《种子》2004年第2期24-26,30,共4页Seed

摘　　要：以 2 5个豌豆品种为材料 ,分别用重蒸水、2 .5 % Na Cl、0 .2 % Na OH和 75 %乙醇提取其种子蛋白 ,利用连续型乳酸 -聚丙烯酰胺凝胶电泳进行分离 ,结果表明 :豌豆贮藏蛋白以水溶、盐溶和碱溶为主 ,醇溶蛋白甚微。三种主要蛋白在品种间存在不同程度的谱带差异 ,均可用于豌豆的品种鉴定 ,但以水溶和碱溶蛋白的谱带差异最大 ,是豌豆品种鉴定的首选蛋白。连续型乳酸 - PAGE方法简便快速、分离效果好 ,是一种经济有效的方法。Seed proteins of 25 pea cultivars were respectively extracted using distilled H 2O, 2.5%NaCl, 0.2%NaOH and 75%alcohol. Based on coherent lactate polyacrylamide gel electrophoresis, the results showed that most of storage proteins were water-soluble, salt-soluble and alkali-soluble proteins. There is a little alcohol-soluble protein. All the three main proteins with polymorphism PAGE-banding patterns can be used in cultivars identification of pea, The salt-soluble and alkali-soluble proteins are more suitable to be used as biochemical marks of pea cultivars than salt-soluble. It also demonstrated that coherent lactate PAGE is a effective method in identification of pea cultivars, which is simple, rapid, inexpensive and has a good effect on protein isolation.

关 键 词：豌豆 品种鉴定 蛋白质电泳 连续型乳酸-聚丙烯酰胺凝胶电泳 分离 

分 类 号：S643.3[农业科学—蔬菜学]