草坪型高羊茅成熟种子胚性愈伤组织诱导及植株再生  

Callus Induction and Plantlet Regeneration from Mature Seeds of Turf-type Tall Fescue

作　　者：余桂红[1] 马鸿翔[1] 佘建明[1] 杨飞武[1] 陆维忠[1] 

机构地区：[1]江苏省农业科学院农业生物遗传生理研究所,江苏南京210014

出　　处：《江苏农业学报》2004年第1期38-43,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业高技术研究项目(BG2003307)

摘　　要：　以5种草坪型高羊茅成熟种子为外植体材料,探讨外植体消毒和不同激素组合对愈伤组织诱导、继代培养以及植株分化的影响。结果表明,不去种皮与去种皮相比,能显著提高高羊茅成熟种子发芽率和出愈率。猎狗5号和野马2号在添加2,4 D10.0mg/L的培养基中愈伤组织诱导率最高;新秀和颂歌的出愈率在2,4 D浓度为8.0mg/L时达到最高;野火的出愈率在2,4 D浓度为4 0～10 0mg/L时变化不大,仅为10.5%～11.2%。用3.0g/L的水晶洋菜(Gelrite)代替琼脂做培养基疑固剂可促进愈伤组织生长及向胚性愈伤组织转化;愈伤组织在含BA2.0mg/L和NAA0.5mg/L的MS培养基上植株分化率较高,其中猎狗5号在此培养基上的愈伤组织植株分化率达72.8%。The mature seeds from five varieties of Festuca arundinacea were used as explants to study the effects of steri-(lization) methods and different hormone compositions on callus induction,proliferation and plantlet regeneration.The results showed the rates of both germination and callus induction were prominently higher in sterilization treatment with seed capsule than in that without seed capsule.The callus induction rates of Houndog5 and Yema2 were the highest on the medium with 10.0 mg/L 2,4-D,while that of Xinxiu and Songge was the highest on the medium with 8.0 mg/L 2,4-D and that of Yehuo didn′t change too much(only increasing 10.5%-11.2%) on the media with 4.0-10.0 mg/L 2,4-D.The proliferation medium with 3.0 g/L gelrite as coagulant instead of 7.0 g/L agar could remarkably promote callus growth and its embryonic transformation.The differentiation medium with 2.0 mg/L BA and 0.05 mg/L NAA was better than the others,and the rate of regeneration of Houndog5 on this medium reached 72.8%.

关 键 词：草坪草 高羊茅 成熟种子 胚性愈伤组织 植株再生 组织培养 转基因 

分 类 号：S688.4[农业科学—观赏园艺]