驻南方部队结核菌株基因分型的流行病学价值研究  

THE EPIDEMIOLOGICAL MEANING OF MYCOBACTERIUM TUBERCULOSIS STRAIN TYPING IN THE ARMY OF SOUTH CHINA

作　　者：曾年华[1] 王志斌[1] 唐博恒[1] 苏建新[1] 姜普林[1] 肖红[1] 王珊珊[1] 李兴国[1] 

机构地区：[1]广州军区联勤部军事医学研究所,广州510507

出　　处：《解放军预防医学杂志》2004年第1期5-9,共5页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：全军青年基金项目 (No .98Q0 2 0 )

摘　　要：目的分析南方部队结核分支杆菌分离株IS 6 110 -RFLPDNA指纹图谱 ,研究菌株指纹分型的流行病学意义。方法首先提取结核分支杆菌基因组DNA ,然后用限制性内切酶PvuⅡ切割 ,琼脂糖凝胶电泳 ,Southern转印后 ,用〔α32 P〕 -dCTP标记的IS 6 110DNA序列中的 2 4 5bp探针杂交 ,放射自显影 ,比较各菌株的IS 6 110拷贝数和带型 ,结合患者一般流行病学资料 ,分析菌株的流行特征。结果共检测分析了 14 3株部队结核分支杆菌DNA的指纹多态性。根据这些菌株的IS6 110DNA指纹多态性特征的同源性共分为 11个类型 ,以Ⅰ型 (34.3% )、Ⅱ型 (2 9.4 % )、和Ⅲ型 (14 .7% )三个型别为主 (78.3% ) ,其余各型均少于 4 %。以 2 0～ 2 9岁和 30～ 39岁组在这三型中所占比例最大 ,分别为 2 7.9%和 2 5 .2 %。初治与复治患者分离菌株的IS 6 110DNA指纹类型的分布有显著性差异 (P <0 .0 1)。所检测菌株是否具有耐药性 ,也有显著性差异 (P <0 .0 5 )。菌株耐药主要为单耐异烟肼或利福平 ,耐药菌株在Ⅰ、Ⅱ、Ⅲ型中所占比例分别是 9.8%、2 .1%和 2 .1%。结论结核分支杆菌IS 6 110 -RFLPDNA指纹分型技术是一种可靠的分型方法 ,可用于结核流行菌株的分子流行病学研究。南方部队结核分支杆菌的传播以Ⅰ、Ⅱ、Ⅲ型为主 。Objective To analyse the IS 6110-restriction fragment length polymorphism(RFLP) DNA fingerprinting patterns of Mycobacterium tuberculosis(MTB) isolated from the army of South China in recent four years,and to study their molecular epidemiolgical characteristics. Methods Extracion of DNA from MTB strains was done by phenol/chloroform method. Then chromosomal DNA was digested with endonuclease Pvu Ⅱ,electrophoresed on agarose gel plate and transferred capillarilly to nylon filter and hybridized by 245 bp fragment of IS 6110 probe labeled with 〔α 32 P〕-dCTP. The RFLP patterns of southern hybridization were detected by autoradiograph method, the relationship between the polymorphism of DNA fingerprinting patterns and epidemiology of MTB were analyzed. Results 143 strains of MTB detected were classified into 11 types according to the homology of IS 6110 DNA fingerprinting. The main types were type Ⅰ(34.3%),type Ⅱ(29.4%),and type Ⅲ(14.7%), and other types were less than 4%. In these main type groups, patients aged from 20 to 29 and 30 to 39 accounted for 27.9% and 25.2% respectively; the distribution of DNA fingerprinting in the first treated patients showed significant difference from that in the retreated patients (P<0.01),and that in the drug-resistant strains was also different (P<0.05). The main drug-resistant strains were isoniazid-resistant or rifampin-resistant strains (9.8%,2.1% and 2.1% respectively in typeⅠ,type Ⅱ and type Ⅲ). Conclusion IS 6100-RELP DNA fingerprinting patterns is a reliable method to analyze MTB, and can be used for the study of molecular epidemiology of tuberculosis. The prevalence of tuberculosis is primarily due to the transmission of type Ⅰ, typeⅡ and typeⅢ in the army of South China, and monitoring for these three types prevalence should be strengthened.

关 键 词：结核分支杆菌 限制性片段长度多态性 分子流行病学 南方部队 

分 类 号：R52[医药卫生—内科学] R181.2[医药卫生—临床医学]