介绍一种可控家兔心肌缺血及再灌注动物模型  

Introduction to a Controllable Myocardial Ischemia-reper fusion Method in the Rabbit

作　　者：陈淑萍[1] 韩振京[1] 刘峻岭[1] 

机构地区：[1]中国中医研究院针灸所生理室,北京市100700

出　　处：《医学理论与实践》2004年第2期125-126,共2页The Journal of Medical Theory and Practice

摘　　要：介绍一种可调控的家兔心肌缺血再灌注模型。选取冠状动脉左室支 (LVB) ,于上 1/ 3处用医用缝合针紧贴 ,经LVB下穿过心肌备用。将丝线两端穿入一小圆的塑料小孔内 (间距约 0 2cm ) ,而后套入一长约 1 2cm ,直径 0 2cm的聚乙稀套管内。当阻塞LVB时 ,将从套管内引出的丝线打单结套住一聚乙稀锥形管。然后 ,将锥形管从细端到粗端缓慢推进 ,直至出现理想的缺血心电图。相反 ,当需要缺血再灌注时 ,将锥形管反方向由粗端到细端抽出 ,使LVB血流重新恢复。这种方法克服了以往一些传统的心肌缺血灌注模型的缺陷 。A controllable acute myocardial isch emia-reperfusion method in the rabbit is introduced After locating the left ve ntricular branc h (LVB) of the coronary artery and the occluded site (upper 1/3), a corneal sutu re needle with a piece of suture (gauge-0, about 10～20cm long) is penetrated be n eath the LVB The two ends of the suture are held to pass through the two holes (about 0 2cm apart) of a thin round plastic space and a polyethylene tube (abo u t 1 2cm long and 0 2cm in diameter) separately When the LVB needs to be occl ude d, the two ends of the suture coming out from the polyethylene tube are made a n ode after placing a plastic conical tube transversely on the top of the polyeth ylene tube Then the conical tube is moved slowly from the thin end to the thic k e n d to cause the suture loop to be tighten till the ideal ischemic electrocardiogr am (ECG) appears When myocardial ischemia-reperfusion needed, the conical tube i s moved in the opposite way to allow the blood flow of LVB to circulate again T his method solves some defects in making myocardial ischemia and reperfusion mod el in the past and is easy to be operated

关 键 词：家兔 心肌缺血 再灌注损伤 动物模型 动物实验 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]