趋化因子MIP-2在大鼠肝缺血/再灌注损伤后的表达  

Expression of macrophage inflammatory protein-2 in liver injury induced by ischemia and reperfusion in rat

作　　者：梁法生[1] 宋继昌[2] 高英堂[2] 张志尧[2] 张文[2] 刘丽川[2] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院肝胆外科,山东烟台264000 [2]卫生部人工细胞工程技术研究中心,天津300170

出　　处：《中国普通外科杂志》2004年第2期104-106,共3页China Journal of General Surgery

摘　　要：目的　研究趋化因子巨噬细胞炎性蛋白 -2 (MIP 2 )在肝缺血 /再灌注损伤中 (I/R )的作用。方法　 3 2只大鼠随机分为 4组 ,每组 8只。即假手术 (对照 )组 ,部分肝脏缺血 90min再灌注 3 ,9,2 4h组。用RT PCR法检测肝组织中MIP 2的mRNA表达 ,ELISA法测定血浆中MIP 2蛋白表达 ,萘酚AS D氯醋酸盐酯酶特染技术检测肝组织内中性粒细胞浸润 ,并测定丙氨酸转氨酶 (ALT )。结果　缺血再灌注肝组织中MIP 2mRNA表达高于非缺血肝组织 (P <0 .0 1) ,9h组高于 3h组 (P <0 .0 1) ,2 4h组高于 9h组 (P <0 .0 1) ;MIP 2血浆蛋白表达、肝内中性粒细胞浸润、ALT也与MIP 2mR NA呈一致性的变化。MIP 2mRNA表达、血浆MIP 2蛋白水平与肝组织中性粒细胞浸润呈正相关 ,(r= 0 .88和 0 .83 ,P <0 .0 1)。结论　MIP 2在大鼠肝缺血 /再灌注损伤中作为中性粒细胞的趋化因子之一起着重要的上调作用。Objective To study the expression and effect of macrophage inflammatory protein-2(MIP-2) in liver injury induced by ischemia/reperfusion(I/R). Methods Thirty-two rats were randomly divided into 4 groups(8 rats in each group):false operation (control) group and 3, 9, 24 hours reperfusion group.The expression of MIP-2 mRNA in hepatic tissue, MIP-2 protein in plasma, the neutrophil infiltration in liver tissue and serum ALT were measured. Results The expression of MIP-2 mRNA in the ischemic tissue was significantly higher than that in nonischemic tissue (P<0.01), and was significantly higher in 9 hours reperfusion group than that in 3 hours reperfusion group (P<0.01); significantly higher in 24 hours reperfusion group than that in 9 hours reperfusion group (P<0.01). The level of plasma MIP-2 protein, the neutrophil infiltration in liver, the degree of liver injury had the similar changs of MIP-2 mRNA.The expression of MIP-2 mRNA in liver ischemia tissue and the level of plasma MIP-2 protein had positive correlation with neutrophil infiltration in liver tissue(P<0.01) . Conclusions The results suggeste that MIP-2, one of the neutrophil chemotactic factors, plays an important role in the liver injury induced by I/R in rats.

关 键 词：肝/血液供给 再灌流损伤 趋化因子 巨噬细胞 

分 类 号：R392.12[医药卫生—免疫学] R619.9[医药卫生—基础医学]