氟对体外培养长骨及成骨细胞影响的电镜观察  

Observation under electron microscope in effects of fluorine on morphosis in culture bone and osteoblast in vitro

作　　者：谢瑶芸[1] 刘开泰[2] 马丽英[2] 李玲芝[3] 张永亮[3] 胡汉华[4] 汤建安[4] 谢凤莲[4] 

机构地区：[1]海南省人民医院电镜室,海口570311 [2]新疆医科大学劳动卫生与环境卫生学教研室,新疆乌鲁木齐830054 [3]天津武警医院,天津300000 [4]新疆医科大学电镜室,新疆乌鲁木齐830054

出　　处：《新疆医科大学学报》2004年第1期15-17,共3页Journal of Xinjiang Medical University

基　　金：国家自然科学基金资助项目 ( 3 0 160 0 79)

摘　　要：目的:探讨氟对体外培养长骨及成骨细胞生长发育、形态结构的影响。 方法:采用体外骨器官及成骨细胞培养实验方法 ,观察不同剂量氟 (0 .0、2 .5、5 .0、10 .0、2 0 .0 μg/ ml)对小鼠胚胎长骨生长发育、形态结构等的影响和不同剂量氟 (0 .0、 10 - 6、10 - 4、10 - 3mg/ m l)对成骨细胞形态的影响。结果 :当染氟剂量≥ 5 .0 μg/ ml,随着染毒剂量的增加 ,尺骨和尺骨骨干形态结构出现明显改变。而当染氟剂量为 10 - 3mg/ m l,成骨细胞超微结构出现明显损害。 结论 :过量的氟对长骨成骨细胞体外培养生长发育、形态结构有明显的影响。s: Objective: To investigate the effects of fluorine on the morphosis in culture bone and osteoblast in vitro. Methods: Mouse fetal were divided into five groups: exposed to 0.0?2.5?5.0?10.0?20.0 μg/ml fluorine and the all fetals were cultured in vitro. and osteoblast,which exposed to 0.0? 10 -6 ?10 -4 ?10 -3 mg/ml fluorine,were cultured The development,morphological changes were determined by electronic and light microscope. Results: At the fluorine concentration were ≥5.0 μg/ml,morphological structure of bone appeared viously abnormal and osteoblast were appearly harm at 10 -3 mg/ml fluorine. Conclusion: It showed that excess fluoride could have adverse effects on the growth and development,morphological structure in culture bone and osteoblast in vitro.

关 键 词：氟 生长发育 形态结构 电镜 

分 类 号：R348.1[医药卫生—基础医学] R329.24