MEK的抑制剂U0126可部分阻断bFGF对辐射诱导的血管内皮细胞凋亡的抑制作用  

MEK inhibitor U0126 may partly block the inhibitory effect of bFGF on radiation-induced apoptosis in endothelial cells

作　　者：谷庆阳[1] 王德文[1] 王立生[1] 邓桦[1] 刘杰[1] 彭瑞云[1] 董波[1] 高亚兵[1] 王晓民[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2004年第1期39-42,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(39470229);全军"九五"医药卫生科研基金课题重点项目(96Z008)资助

摘　　要：为研究 bFGF(碱性成纤维细胞生长因子)对辐射诱导的血管内皮细胞凋亡的作用及其信号转导途径,以 Coγ 射线照射法建立人脐静脉内皮细胞株 ECV304 细胞凋亡模型,以流式细胞术(Annexin-V-FITC+PI 标 60记)检测细胞凋亡率。观察不同浓度的 bFGF 和 MEK 的特异性抑制剂 U0126 对细胞凋亡的影响。结果显示,bFGF 对辐射诱导的 ECV304 细胞凋亡有明显的抑制作用,而 U0126 可阻断 bFGF 的抗凋亡作用。提示 bFGF可能通过 MAPK 信号转导途径抑制辐射诱导的血管内皮细胞凋亡。In order to investigate the effect of bFGF on radiation-induced apoptosis in vascular endothelial cells and its signal transduction pathways, ECV304 cells were irradiated with Co γ rays for establishing cell apoptosis 60 model. Flow cytometry with Annexin-V-FITC-PI labeling was used to determine cell apoptosis. Different amounts of bFGF and MEK inhibitor U0126 were used to study their effects on radiation-induced endothelial cell apoptosis. It was found that bFGF could inhibit radiation-induced apoptosis in ECV304 cells, but U0126 could block the anti-apoptosis effect of bFGF, suggesting that bFGF may inhibit radiation-induced apoptosis in vascular endothelial cells through of MAPK signaling.

关 键 词：MEK 血管内皮细胞 细胞凋亡 抑制剂 BFGF 辐射诱导 碱性成纤维细胞生长因子 细胞培养 

分 类 号：R818.71[医药卫生—放射医学]