防治菜青虫的杀虫植物筛选及活性的初步研究  被引量:14

The Pilot Studies on the Screening and Bioactivity of Insecticidal Plants Against Pieris rapae(L)

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作  者:蒲玮[1] 肖波[1] 张可君[1] 侯太平[1] 

机构地区:[1]四川大学生命科学学院,成都610064

出  处:《四川大学学报(自然科学版)》2004年第1期184-188,共5页Journal of Sichuan University(Natural Science Edition)

基  金:国家自然科学基金(29902006);四川省应用基础项目(01NY051 02)

摘  要:测试了25种有毒植物的95%乙醇提取物对3龄菜青虫的毒杀活性.结果表明,瑞香狼毒、巴豆对菜青虫具有较好的防治效果.在此基础上,以正己烷、氯仿、乙酸乙酯为溶剂对狼毒、巴豆的无水乙醇提取液作分级萃取,所得萃取液的生物活性测试结果表明:巴豆及狼毒的正己烷萃取物对菜青虫有较强毒杀作用和拒食活性,当样品稀释倍数为50倍,其48h内校正死亡率分别为88.9%和66.7%,72h内校正死亡率则分别达到95.6%和91.1%,72h的平均拒食率则分别达到87.5%和83.3%.The 95% ethanol extracts from 25 poisonous plant species were tested for the poison activity against 3rd instar larvae of Pieris rapae(L).The results showed that the extracts from Stellera chamaijasme L. and Croton tiglium L. had fairly good control effect. On that base, the author made gradient extraction from the anhydrous ethanol extracts of the two plants with three organic solvent including : n-hexane?chloroform and ethyl acetate. Through the insecticidal activity test of the extracts mentioned above, found out the n-hexane extract from Stellera chamaijasme and Croton tiglium had significant stronger poison activity and antifeedant activity against Pieris rapae. When diluted the test specimen at a concentration of 50 times dilution respectively, the death rates of the insects in 48h were 88.9% and 66.7%,in 72h reached as high as 95.6% and 91.1%, and the average antifeedant rates in 72h reached to 87.5% and 83.3%.

关 键 词:菜青虫 植物杀虫剂 生物活性 

分 类 号:Q946[生物学—植物学]

 

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