垂吊型矮牵牛叶片培养不定芽发生和微繁研究  

ADVENTITIOUS SHOOT REGENERATION AND MICROPROPAGATIONFROM LEAVES OF TRAILING PETUNIA(Petunia hybrida)

作　　者：臧运祥[1] 张松[1] 郑伟尉[1] 陈美霞 达克东[1] 吴禄平[3] 

机构地区：[1]山东农业大学园艺学院,山东泰安271018 [2]山东潍坊职业技术学院,山东潍坊250086 [3]沈阳农业大学园艺学院,辽宁沈阳110161

出　　处：《核农学报》2004年第1期18-21,共4页Journal of Nuclear Agricultural Sciences

摘　　要：以垂吊型矮牵牛叶片为材料 ,建立了垂吊型矮牵牛叶片高效不定芽发生、植株再生和微繁的技术体系。研究了不同激素及其组合对叶片不定芽发生、壮苗和生根的效应。结果显示 ,MS +BA 2mg L +NAA 0 1mg L是叶片不定芽发生的适宜培养基 ;MS +BA 0 5mg L +IBA0 1mg L +GA 0 5mg L是不定芽形成壮苗的适宜培养基 ;MS +IAA 0 1mg L是壮苗生根的适宜培养基 ;High frequent adventitious shoot formation, plant regeneration and micro-propagation were established in trailing petunia(Petunia hybrida) leaf. Effect of different kind of hormone and its combination on adventitious shoot formation, plant regeneration and root regeneration was studied. The result showed that MS+BA 2mg/L+NAA 0.1mg/L was suitable for adventitious shoot formation from the leaf, the shoots grew well in MS+BA 0.5mg/L+IBA 0.1mg/L+GA 0.5mg/L medium, MS+IAA 0.1mg/L was the best medium for root regeneration. Turfysoil combined with perlite (1:1) was suitable for the growth of transplanted plantlet.

关 键 词：垂吊型矮牵牛 叶片培养 不定芽 微繁殖 组织培养 激素配比 培养基 

分 类 号：S681[农业科学—观赏园艺]