氧化剂对RAW264.7细胞M-CSF基因表达的调控  

Oxidant regulates macrophage colony-stimulating factor gene expression in RAW264.7 cells

作　　者：庞战军[1] 周玫[2] 陈瑗[2] 

机构地区：[1]第一军医大学南方医院妇产科,广东广州510515 [2]第一军医大学自由基医学研究室,广东广州510515

出　　处：《中国病理生理杂志》2001年第1期9-12,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No .3 9670 197)

摘　　要：目的 :揭示氧化应激对巨噬细胞巨噬细胞集落刺激因子 (M -CSF)基因表达的影响。方法 :采用逆转录多聚酶链反应 (RT -PCR)技术 ,观察氧化剂叔丁基氢过氧化物 (tbOOH)对RAW2 64 7细胞M -CSFmRNA表达的影响。结果 :一定浓度的tbOOH( 1 5× 10 -4mol/L)能够诱导RAW2 64 7细胞M -CSFmRNA表达 ,且随tbOOH浓度的增加 ,其对M -CSFmRNA的诱导作用增强 ;另外 ,环己酰亚胺 (cycloheximide)及acetovanilone可减弱tbOOH对RAW2 64 7细胞M -CSF表达的诱导。结论 :tbOOH可诱导RAW2 64 7细胞M -CSFmRNA的表达 ,且作用在转录水平 ,该过程中有新的蛋白质合成及内源性活性氧产生的参与。AIM:To find out the effect of oxidative stress on macrophage colony-stimulating factor (M-CSF) gene expression in macrophages.METHODS: The effect of tert-butyl hydroperoxide (tbOOH) on M-CSF expression in RAW264.7 cells was investigated by RT-PCR method.RESULTS: The results showed that tbOOH (1 5×10 -4 mol/L) could induce M-CSF mRNA expression in RAW264.7 cells; and the induction became greater with increase in tbOOH concentration. Furthermore, the induction of M-CSF in RAW264.7 cells by tbOOH could be attenuated by cycloheximide or acetovanilone added in the medium.CONCLUSION: The results indicated that tbOOH could transcriptionally induce M-CSF expression in RAW264.7 cells, and de novo protein synthesis and intracellular superoxide (O  2) production might be involved in the process.

关 键 词：巨噬细胞集落刺激因子 氧化剂 细胞系 

分 类 号：Q786[生物学—分子生物学]