前列腺分泌蛋白94基因的克隆及序列测定  

Clone and sequence analysis of prostate secretory protein of 94 amino acids

作　　者：田长生[1] 高瑞娟[1] 李春凌[1] 赵雪俭[1] 刘树铮[2] 

机构地区：[1]白求恩医科大学病理生理教研室,吉林长春130021 [2]白求恩医科大学预防医学院,吉林长春130021

出　　处：《中国病理生理杂志》2001年第1期19-22,共4页Chinese Journal of Pathophysiology

基　　金：吉林省科技发展计划项目 (No.2 0 0 0 1110 ) ;JICA(日本国际协力事业团 )资助项目

摘　　要：目的 :作为构建前列腺分泌蛋白 94 (PSP94 )分泌型表达质粒的基础。方法 :从正常前列腺组织中钓取PSP94cDNA ,构建重组质粒pUC19-PSP94 ,转化后提取目的基因并进行测序。结果 :( 1)构建了pUC19-PSP94的重组克隆质粒 ;( 2 )PCR扩增获得了 2 80bp的PSP94成熟蛋白基因 ;( 3 )测定了PSP94的基因序列。结论 :所构建的pUC19-PSP94重组质粒可成功地克隆PSP94蛋白基因。AIM: To construct recombinant human prostate secretory protein of 94 amino acids(PSP94) expression vector. METHODS: The PSP94 cDNA was obtained from normal prostate tissue, and recombinant plasmid pUC19-PSP94 was constructed. The target gene was identified and sequenced. RESULTS: 1) The recombinant plasmid pUC19-PSP94 was constructed; 2) The mature PSP94 protein gene of 280bp was acquired by PCR; 3) The gene sequence of PSP94 was identified.CONCLUSION: The constructed plasmid pUC19-PSP94 could to be clone PSP94 successfully.

关 键 词：前列腺 分泌 蛋白质类 基因 质粒 

分 类 号：Q784[生物学—分子生物学]