“一肾一夹”高血压大鼠肾脏皮质钠泵α亚单位的基因表达  

Isoform-specific regulation of sodium pump α-subunit gene expression in the cortex of kidney from the one kidney one clip hypertensive rat

作　　者：原卫清[1] 王颢[1] 吕卓人[1] 张明娟[1] 杨军[2] 

机构地区：[1]西安交通大学第一医院心内科,陕西西安710061 [2]西安交通大学第二医院病理科,陕西西安710061

出　　处：《中国病理生理杂志》2001年第1期46-49,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨“一肾一夹 ( 1k1c)”高血压大鼠肾脏皮质钠泵α亚单位各异构体基因表达的改变 ,为高血压发病机制的深入研究提供理论及实验依据。方法 :分别应用RT -PCR及免疫组化技术 ,探讨 1k1c高血压大鼠肾脏皮质钠泵α1、α2 及α3亚单位mRNA及蛋白水平基因表达的改变。结果 :正常大鼠肾脏皮质主要表达钠泵α1亚单位 ,α2 与α3亚单位表达较弱 ;在mRNA水平 ,1k1c高血压大鼠肾脏皮质钠泵α1亚单位表达明显较强 ,α2 与α3亚单位表达无改变 ,而在蛋白水平各亚单位基因表达与对照组比较均无明显差异。结论 :1k1c高血压大鼠肾皮质钠泵α亚单位基因表达发生改变 ,这种改变可能参与该模型的血压升高机制。AIM: To investigate the regulation of sodium pump α-subunit gene expression in the cortex of kidneys from one kidney one clip (1k1c) hypertensive rats. METHODS: 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney. 4 weeks later, all the rats were killed, the levels of sodium pump α 1-, α 2-, and α 3-subunit mRNA and protein in the cortex of kidney were detected with reverse transcription polymerase chain reaction (RT-PCR) method and immunohistochemical assay, respectively. RESULTS: The mRNA levels of sodium pump α 1-subunit increased and α 2- and α 3-subunit were unchanged in the cortex of kidneys from 1k1c hypertensive rats compared with control rats, while no change has been found for all the three subunits gene expressions at protein level. CONCLUSION: There were some changes in the expression of sodium pump α-subunit gene in the cortex of kidney of the 1k1c hypertensive rat, which might be related to the development of hypertension in this hypertensive model.

关 键 词：高血压 大鼠 肾皮质 钠钾泵 基因表达 

分 类 号：Q344.13[生物学—遗传学] R544.1[医药卫生—心血管疾病]