用随机扩增多态DNA(RAPD)技术鉴定野山人参  

RAPD Differentiation of wild Ginseng Species

作　　者：丁建弥 万树文 梅其春[2] 邵永平[2] 

机构地区：[1]上海雷允上药业有限公司神象参茸分公司,上海200002 [2]复旦大学生命科学学院,上海200433

出　　处：《中成药》2001年第1期3-5,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :探索用随机扩增多态DNA(RAPD)技术鉴别野山参和栽培人参的可能性。方法 :从经过形态鉴定的野山人参和栽培人参药材中提取DNA模板 ,运用RAPD技术在电泳中产生条带。结果 :用 80个引物 ,其中一个引物产生野山人参可重复的特征条带。结论Objective: To explore the potentiality of random amplified polymorphic DNA (RAPD) technique for the differntiation of wild and cultivated Ginseng.Methods: DNA templated were extracted from wild and cultivated Ginseng of the crude drugs that were identified by morphology, and RAPD technique was applied to produce electrophoresis pattern. Results: Among the 80 primers screened, one was able to generate reproducible band, characteristic RAPD marker band which was unique to each of wild Ginseng samples was identified. Conclusion: The results demonstrated that RAPD marker technique could effectively authenicate wild and cultivated Ginseng samples.

关 键 词：RAPD 野山人参 栽培人参 

分 类 号：R917[医药卫生—药物分析学]