HPLC测定戊己丸中盐酸小檗碱和盐酸巴马汀的含量  

Determination of Berberiue hydrochloride and Palmatine hydrochloride in Wuji Pills by HPLC

作　　者：金佩芬[1] 缪华蓉[1] 钱亚萍[1] 崔波[1] 

机构地区：[1]嘉兴市药品检验所,浙江嘉兴314001

出　　处：《中成药》2001年第1期24-25,共2页Chinese Traditional Patent Medicine

摘　　要：目的 :建立戊己丸中盐酸小檗碱和盐酸巴马汀的含量测定项。方法 :超声提取 ,使用C18柱 ,0 .0 33mol/L磷酸二氢钾溶液 乙腈 (90∶40 )为流动相 ,检测波长为 2 6 5nm。结果 :方法线性关系良好 ,平均回收率 ,盐酸小檗碱为 99.14% ,盐酸巴马汀为 98.5 9% ;RSD分别为 1.3%和 1.7%。结论 :本方法分离良好 。Objective: To establish the method of determination of the content of Berberine hydrochloride and Palmatine hydrochloride in Wuji Pills. Methods: Ultrasonic extraction, HPLC was used to determine Berberine hydrochloride and Palmatine hydrochloride in Wuji Pills on C 18 Column, using 0.033mol/L KH 2PO 4 CH 3CN(90∶40) as mobile phase, with detection at 265nm. Results: The linearity of this method was well. The average recoveries were 99.07% for Berberine hydrochloride and 98.59% for Palmatine hydrochloride. RSD were 1.3% and 1.7%, respectively. Conclusion: This method shows good separating degree and can be used for quality control of Wuji Pills.

关 键 词：高效液相色谱 盐酸小檗碱 盐酸巴马汀 戊己丸 

分 类 号：R284.1[医药卫生—中药学]