激光二极管泵浦激光诱导荧光检测装置的研究  

A Laser-induced Fluorescence Detection System with a Laser Diode Double-pumped Solid State Laser as the Excitation Source

作　　者：杨丙成[1] 谭峰[1] 陈令新[1] 关亚风[1] 

机构地区：[1]中国科学院大连化学物理研究所微型仪器与分析化学研究室,辽宁大连116012

出　　处：《分析测试学报》2004年第1期91-93,共3页Journal of Instrumental Analysis

基　　金：科技部"十五"科学仪器攻关资助项目 (011806)

摘　　要：基于共聚焦光学结构 ,以发射波长532nm的二极管泵浦固体激光器为激发光源研制了一小型荧光检测器 ;以罗丹明B为荧光试剂 ,柱上检测模式评价了该检测体系 ,焦宁Y为背景荧光探针间接检测了无机金属离子 ,结果表明该系统具有体积小、成本低、高灵敏度和易操作等优点。Aportable laser-induced fluorescence detector based on confocal configuration is described,usˉing a laser-diode double-pumped solid-state laser(LD-DPSSL)as the excitation source.On-colˉumn detection modes with Rhodamine B were employed for the evaluation of the LIF-D system.Indirect fluorescence detection of mental cations using Pyronine Yas the background probe was performed.The reˉsults showthat this systemhas the advantages of small size,lowcost,high sensitivity and easy operation.

关 键 词：激光二极管泵浦固体激光器 激光诱导荧光检测 共聚焦 柱上检测模式 焦宁Y 无机金属离子 

分 类 号：O657.319[理学—分析化学]