里氏木霉(Trichoderma reesei)306产组织型纤溶酶原激活剂(t-PA)液体发酵条件的优化  

Optimization of Tissue-type Plasminogen Activator (t-PA) Production using Trichoderma reesei 306

作　　者：冮洁[1] 杜连祥[1] 路福平[1] 邹亚杰[2] 贾丹丹[2] 姚蕾[2] 

机构地区：[1]天津科技大学,天津300222 [2]齐齐哈尔大学,齐齐哈尔161006

出　　处：《食品与发酵工业》2004年第1期29-33,共5页Food and Fermentation Industries

基　　金：黑龙江省自然科学基金资助项目 (No.D0 3 5 2 )

摘　　要：采用正交设计和响应面分析等实验方法对基因工程菌株里氏木霉 (Trichodermareesei)30 6产组织型纤溶酶原激活剂 (t PA)的液体发酵条件进行了优化。确定了适宜的培养基配方和最佳发酵工艺条件。在优化发酵条件下 ,摇瓶液体发酵液中的t PA酶活力达 3386 91IU/mL ,比初始发酵条件下酶活力提高上千倍。The fermentation conditions of tissue-type plasminogen activator (t-PA) producing using recombinant Trichoderma reesei 306 was studied. Through orthogonal experiment and response surface methods, the optimum medium and the optimum fermentation condition were determined. The optimized medium is: bran 31 g/L, urea 14.5 g/L, tryptone 0.2 g/L, dissolubility cellulose 20 g/L, KH 2PO 4 15 g/L, MgSO 4·7H 2O 1.18 g/L, MnSO 4·H 2O 0.0013 g/L, CaCl 3 2H 2O 0.8 g/L, Tween 80 lg/L. The t-PA yield of 3386.91 IU/mL was obtained at the intial pH of 5.4, temperature of 28℃, rotation speed of 150 r/min for 36h in a 250mL flask with 50mL broth, which is over one thousand times than control.

关 键 词：里氏木霉 组织型纤溶酶原激活剂 液体发酵 培养基配方 工艺优化 

分 类 号：TQ464[化学工程—制药化工]