大鼠急性全脑缺血再灌注损伤后细胞凋亡及红花保护作用的研究  

Research of apotosis after acute cerebral ischemia reperfusion in rat brain and safflower protection mechanism

作　　者：赵晴[1] 杜建时[2] 韩雪梅[1] 徐凤梅 杨昕[1] 康治臣[1] 李红 

机构地区：[1]吉林大学中日联谊医院神经科,吉林长春130033 [2]吉林大学中日联谊医院血管外科 [3]辽源市西安区人民医院 [4]吉林省洮南县神经精神病院

出　　处：《中国实验诊断学》2004年第1期21-23,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨红花在大鼠全脑缺血再灌注损伤中的脑保护作用及其分子机制。方法　选用Wistar大鼠 70只 ,随机分为红花组 ,盐水对照组 ,假手术组 ,单纯缺血组。制备大鼠全脑缺血再灌注损伤模型。通过光镜、电镜、原位杂交及免疫组化方法测定Caspase 3、Bcl 2在脑缺血再灌后不同时间蛋白表达的变化 ,并用图像分析方法测定其免疫强度。结果　红花与盐水对照组相比使相应时间点Caspase 3表达降低 ,Bcl 2表达升高。结论　红花可减轻脑缺血再灌注损伤 ,具有脑保护作用。Objective To explore the protections and molecular mechanism of safflower in rat cerebral ischemia reperfusion. Methods 70 Wistar rats were randomly allocated to four groups:safflower group, normal saline control group, sham-operation group, ischemia-only group. To make the model of global cerebral ischemia reperfusional injury rat. By light microscope, electron microscope, immunohistochemical method and hybridization in situ technique to observe the expression of Bcl-2 and Caspase-3 after cerebral ischemia reperfusion varing with time and to measure the expression levels of Bcl-2 and Caspase-3 by graphic analysis.Results The expression of Caspase-3 is lower and Bcl-2 is higher in safflower group than those in normal saline control group at the same time. Conclusion Safflower can alleviate the injury of global cerebral ischemia reperfusion.

关 键 词：大鼠 急性全脑缺血 再灌注损伤 细胞凋亡 红花 脑保护作用 Caspase-3 BEL-2 脑血管病 

分 类 号：R285[医药卫生—中药学]