人可溶性CD14检测体系的建立  

Establishment of a system for determination of human soluble CD14

作　　者：江海燕[1] 刘艳君[1] 朱平[1] 韩强涛[1] 富宁[1] 

机构地区：[1]第一军医大学免疫教研室,广东广州510515

出　　处：《中国实验诊断学》2004年第1期34-37,共4页Chinese Journal of Laboratory Diagnosis

基　　金：广东省自然科学基金资助 ( 0 0 10 5 8)

摘　　要：目的　建立人可溶性CD14双抗体夹心ELISA检测体系。方法　用抗人CD14单抗为捕获抗体 ,加入待测抗原 ,第二抗体为兔抗人CD14多克隆抗体 ,最后加入辣根过氧化物酶标记的羊抗兔IgG。结果　本检测体系灵敏度高 ,可达 5ng ml,特异性强 ,测量范围 5～ 12 0ng ml,重复性好 ,各项技术指标与国外同类产品相当。结论　本检测体系能够检测人体液中和细胞培养上清中sCD14 ,适用于临床及基础研究。Objective To establish a sandwich ELISA for the determination of soluble CD14 in human body fluid. Methods An anti-CD14 monoclonal antibody was coated on polystyrene microtiter plates; then samples for detection were added to the wells; followed by anti-human CD14 polyclonal antibody prepared from rabbit; at last, addition of HRP-goat anti-rabbit IgG completed the sandwich ELISA. Results The specificity of this sCD14 ELISA is high, the lowest detectable level is 5ng/ml, the range of detection is 5ng/ml～120ng/ml. Conclusion This sCD14 ELISA is applicable for research and observation in clinical.

关 键 词：可溶性CD14 双抗体夹心ELISA法 多克隆抗体 抗原 细胞免疫 血清 

分 类 号：R446.6[医药卫生—诊断学]