生物碳素材料表面血小板黏附的实验研究  

Probe into the Platelets Adhesion to Carbonaceous Biomaterials

作　　者：李伯刚[1] 那娟娟[1] 尹光福[1] 殷杰[1] 郑昌琼[1] 

机构地区：[1]四川大学材料科学与工程学院,成都610065

出　　处：《生物医学工程学杂志》2004年第1期12-15,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 ( 3 9870 2 3 2 );教育部博士点基金资助项目 ( 2 0 0 0 610 0 4)

摘　　要：为了解生物碳素材料的凝血机制 ,将新鲜抗凝人血离心分离为富血小板血浆 ,在 37℃恒温条件下 ,对类金刚石薄膜 (DL C)、金刚石薄膜和石墨三种碳素材料进行了血小板黏附实验 ,通过扫描电镜对黏附于材料表面的血小板进行形态观察和计数分析 ,用形态指数描述血小板的变形程度。结果显示 ,DL C表面无血小板黏附 ,而金刚石薄膜和石墨表面均黏附有为数不少、呈 ～ 型重度变形的血小板。血小板的黏附量石墨最多 ,而形态指数则金刚石薄膜更大。经与前期研究和文献报道的对比分析 ,得出三个主要结论 :(1)蛋白吸附介导的血小板黏附、变形和聚集是生物碳素材料的主要凝血机制 ;(2 )评价生物碳素材料的血液相容性 ,血小板变形度比血小板消耗率更有价值 ;(3) DL C的纯度越高 ,血液相容性越好。In order to clarify the mechanism of blood coagulation for carbonaceous biomaterials,the plasma rich in platelet was obtaining through the centrifugation of fresh human blood containing anticoagulant. Adhesive tests of platelets to surfaces of DLC,diamond film(DF) and graphite was carried out at 37℃.Then,morphology observation,counting and deformation index calculation of the platelets adhering to surfaces of the three kinds of materials were analyzed by SEM. It has been shown that there is no any platelet on the surface of DLC,but on DF and graphite,a lot of platelets are observed with serious deformation of type Ⅲ～Ⅴ.The adhesive amounts of platelet on the surface of graphite are more than those on DF,but deformation index of platelets on the surface of DF is more than that on graphite. Three major conclutions have been obtained through comparative analyses with our previous researches and related literatures: (1) Adhesion,deformation and collection of platelets occoured in succession on material surfaces resulting from protein adsorption are the major mechanism of blood coagulation of carbonaceous materials; (2) Deformation degree of platelets is more important hemocompatibility index than consumption ratio of platelets for carbonaceous materials; (3) The purer the DLC,the better is the hemocompatibility. These conclusions possess important directive function for improving and designing carbonaceous materials used in artificial mechanical heart valves.

关 键 词：生物碳素材料 血小板黏附 金刚石薄膜 石墨 血液相容性 

分 类 号：R318.08[医药卫生—生物医学工程]