生物材料溶血性标准化评价方法比较:溶血率法和氰化高铁血红蛋白法  

Comparison between Hemolysis Percentage Measurement and Hemiglobincyanide Measurement for Standardizing the Evaluation of Hemolytic Properties of Biomaterials

作　　者：张伶俐[1] 朱蔚精[1] 谭言飞[1] 屈树新[1] 张兴栋[1] 

机构地区：[1]四川大学分析测试中心生物学评价实验室,生物材料工程研究中心成都610064

出　　处：《生物医学工程学杂志》2004年第1期111-114,137,共5页Journal of Biomedical Engineering

摘　　要：采用游离血红蛋白直接测定法即溶血率测定法 (根据 ISO/ TR74 0 5 )和氰化高铁血红蛋白法 (根据 ISO10 993- 4 )测定了羟基磷灰石 (陶瓷 )和胶原 (高分子材料 )的溶血性能 ,结果提示这两种方法的结论是一致的 ,且氰化高铁血红蛋白法较血红蛋白直接测定法有灵敏、稳定、可比性好等优点 ,值得推广应用。但它存在着一些标准中尚未明确规定的问题。作者在大量试验和不违背标准原则的基础上提出了一些改进意见 ,使它更具有可操作性 。In this study, two methods——the supernatant hemoglobin spectrophotometry, i.e. hemolysis percentage measurement(according to ISO/TR 7405) and the hemiglobincyanide measurement(according to ISO 10993-4)——were used to assay the hemolytic properties of hydroxyapatite(bioceramics) and collagen (polymer). The results showed that the conclusions drawn from using the two methods were basically consistent, and the latter was more sensitive, stable and comparable. However, some of the procedures in the hemiglobincyanide method were not defined in details. So based on our experiments we have offered some suggestions and improvements, which do not deviate from ISO and ASTM standards, for hiher practicability of usig it in standardizing the evaluation of the hemolytic properties of biomaterials. Hemiglobincyanide measurement is worthy of wider application.

关 键 词：生物材料 溶血性标准化 溶血率法 氰化高铁血红蛋白法 血液相容性 溶血试验 生物学 

分 类 号：R318.08[医药卫生—生物医学工程]