重组海葵溶细胞素及平颏海蛇磷脂酶A_2对血管外膜成纤维细胞增殖的影响  

Effects of recombinant Sagartia rosea cytolysin and Lapemis hardwickii phospholipase A_2 on adventitial fibroblasts proliferation

作　　者：欧阳平[1] 姜孝玉[2] 杨文利[2] 赖文岩[1] 徐安龙[2] 

机构地区：[1]第一军医大学南方医院心血管内科,广东广州510515 [2]中山大学生命科学学院国家高技术计划海洋生物功能基因组开放实验室,广东广州510275

出　　处：《第一军医大学学报》2004年第2期158-160,共3页Journal of First Military Medical University

基　　金：国家863计划项目(2001AA628090);广州市科委科技攻关引导项目(2002z3-C7111)~~

摘　　要：目的观察重组海葵溶细胞素(rSrc)及平颏海蛇磷脂酶A2(rPLA2)对成纤维细胞增殖的影响。方法体外培养成纤维细胞,应用不同浓度的rSrc和rPLA2分别进行作用并设立对照进行比较,采用非放射性的MTS/PES法确定成纤维细胞的增殖状态。结果各组细胞增殖率分别为:对照组0.840±0.061、rSrc 100 μg/ml组0.263±0.044、rSrc 10 μg/ml组0.418±0.054、rSrc 1 μg/ml组0.605±0.063、rSrc 100 ng/ml组0.772±0.054、rSrc 10 ng/ml组0.906±0.072、rPLA2 100 μg/ml组0.498±0.076、rPLA2 10 μg/ml组0.937±0.112、rPLA2 1 μg/ml组0.978±0.145。统计分析显示,低至1 μg/ml的rSrc仍能明显抑制大鼠成纤维细胞的增殖(P<0.01),并呈现剂量依赖性(P<0.05)。rPLA2 100 μg/ml组与对照组相比细胞增殖率有显著差异(P<0.05);rPLA2 10 μg/ml和rPLA2 1 μg/ml组与对照组相比均无显著差异(P>0.05)。结论rSrc和rPLA2分别对血管外膜成纤维细胞增殖有一定的抑制作用。Objective To observe the effects of recombinant Sagartia rosea cytolysin (rSrc) and Lapemis hardwickii phospholipase A2(rPLA2) on adventitial fibroblasts proliferation. Methods NIH-3T3 cells were cultured and treated with rSrc and rPLA2 at different concentrations for observation of cell proliferation using non-radioactive MTS/PES assay in comparison with the control group. Results The ratio of cell proliferation was 0.840±0.061 in the control group, and was 0.263±0.044, 0.418±0.054, 0.605±0.063, 0.772±0.054 and 0.906±0.072 in rSrc groups corresponding to rSrc concentrations of 100, 10, 1 μg/ml and 100, 10 ng/ml respectively. rSrc was found to significantly inhibit fibroblast proliferation in a dose-dependent manner when the concentration used was above 1 μg/ml (P<0.05), as compared with the control group (P<0.05). The ratio of cell proliferation was 0.498±0.076, 0.937±0.112 and 0.978±0.145 in rPLA2 groups corresponding to rPLA2 concentrations of 100, 10, 1 μg/ml respectively, indicating that rPLA2 also significantly inhibited fibroblast proliferation at the concentration of 100 μg/ml (P<0.05). Conclusion rSrc and rPLA2 can both significantly inhibit adventitial fibroblast proliferation.

关 键 词：重组海葵溶细胞素 平颏海蛇磷脂酶A2 血管外膜成纤维细胞 细胞增殖 抑制作用 

分 类 号：R543[医药卫生—心血管疾病]