猪多杀性巴氏杆菌PCR检测方法的建立及其应用  

Establishment and application of PCR method of detection for swine Pasteurella multocida

作　　者：唐先春[1] 吴斌[1] 陈焕春[1] 

机构地区：[1]华中农业大学畜牧兽医学院动物病原微生物实验室,湖北武汉430070

出　　处：《中国兽医科技》2004年第2期46-48,共3页Chinese Journal of Veterinary Science and Technology

基　　金：湖北省重点科技攻关项目 (2 0 0 1AA2 0 1)

摘　　要：设计了 1对用以检测猪多杀性巴氏杆菌的引物。对临床送检的有肺炎症状或萎缩性鼻炎症状的发病猪的病料进行细菌培养 ,然后挑菌做PCR ,同时对可疑菌落纯化培养后做生化鉴定。结果 ,从不同省 (市 )的送检病料中分离鉴定出 6 6株多杀性巴氏杆菌 ,PCR检测结果与生化鉴定结果完全符合 ;该引物对其他 6种猪的常见呼吸道病原菌的扩增结果均为阴性 ;该PCR检测方法能检出CFU为 10 3/mL的巴氏杆菌模板。A set of specific primers to amplify 457 bp DNA product was designed to detect swine Pasteurella multocida. The lung tissues or nasal swabs from pneumonic swine and atrophic rhinitis swine were streaked on TSA, then the bacteria clones were detected by the PCR and by the biochemical reaction after the bacteria were purified. With the mentioned-above methods, 66 strains of Pasteurella multocida were (isolated) from the different areas. The PCR result was quite coincident with that of the biochemical reaction. The detection results of 6 other swine respiratory pathogenic bacteria were negative by the set of primers. CFU 1×10~3/mL Pasteurella multocida template can be detected by the PCR.

关 键 词：多杀性巴氏杆菌 PCR 生化鉴定 猪 

分 类 号：S858.28[农业科学—临床兽医学] S852.616[农业科学—兽医学]