高蛋白高胆固醇饮食诱导大鼠心肌纤维化协同效应及其机制的研究  

THE COORDINATED EFFECT OF THE EXCESSIVE PROTEIN AND CHOLESTERIN INTAKE ON INDUCING RAT MYOCARDIAL FIBROSIS AND ITS MECHANISM

作　　者：谢晓华[1] 李朝晖 陈雯[1] 卢文宁[1] 刘宁[2] 刘秀华[1] 唐朝枢[3] 

机构地区：[1]解放军总医院,北京100853 [2]天津解放军第272医院,天津300020 [3]北京大学医学部,北京100083

出　　处：《中国应用生理学杂志》2004年第1期43-45,共3页Chinese Journal of Applied Physiology

基　　金："十五"军队医药卫生科研基金重点课题资助(0 2Z0 10 )

摘　　要：目的 :探讨高蛋白高胆固醇饮食诱导心肌纤维化的协同效应及其发生机制。方法 :在每日标准饮食中增加2 0 %蛋白质或 /和 10 0mg胆固醇摄入 8周的大鼠 ,以羟脯氨酸法测心肌胶原含量 ;以放免法测左心室及血浆AngⅡ和Ald浓度 ;以Griess法测血清亚硝酸盐 (NO-2 )浓度。结果 :高蛋白高胆固醇组较高蛋白组心肌胶原含量升高了1.6 9倍 ,血总胆固醇和AngⅡ浓度分别升高了 0 .7倍和 1.5倍 ,血NO-2 浓度亦明显降低 ,心肌Ald含量上升了 1倍 ;较高胆固醇组心肌胶原含量升高了 0 .4 8倍 ,血AngⅡ升高了 0 .2 3倍。 结论 :高蛋白高胆固醇饮食可协同诱导心肌纤维化 ,其发生机制可能与RAAS激活和内皮功能受损有关。Aim: To investigate the coordinated role and its mechanism of the high protein and hypercholesterin intake on inducing rat myocardial fibrosis. Methods: The tissue level of the collagen in left ventricule,the concentrations of the plasma and the cardiac tissue angiotensin Ⅱ(Ang Ⅱ) and Aldosterone(Ald),the serum concentration of nitrite(NO - 2),in the wister rats on diet which adding 20% protein or/and 100 mg/d cholesterin in the rat standard foods for 8 weeks,were measured by the colorimetric analysis of the hydroproline,by the radioimmunoassay,and by the assay of Griess,respectively. Results: 1.69 times left ventricular collagen contents,0.7 times plasma concentrations of total cholesterin,1.5 times levels of the plasma AngⅡand 1 time myocardial ald contents were higher,and the serum NO - 2 concentration was significant lower,in the rats of the high protein and hypercholesterin intake than in the rats of the high protein intake.That 0.48 times left ventricular collagen contents,0.23 times plasma AngⅡ in the high protein and hypercholesterin intake rats were higher than in the high cholesterin intake rats. Conclusion: The excessive protein and cholesterin intake can induce the coordinated effect on developing the myocardial fibrosis of rats. And the mechanism of the fibrosis in rat left venticule maybe result with the activation of RAAS and the endothelial injury.

关 键 词：高蛋白高胆固醇饮食 心肌纤维化 RAAS 一氧化氮 

分 类 号：R542.23[医药卫生—心血管疾病]