一种食用菌提取物y3对烟草花叶病毒的钝化作用及其机制  

Study on inactivation of Tobacco mosaic virus by y3 from Mushroom (Coprinus comatus)

作　　者：吴丽萍[1] 吴祖建[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所,福建福州350002

出　　处：《中国病毒学》2004年第1期54-57,共4页Virologica Sinica

基　　金：教育部骨干教师资助项目(2000-65);福建科委重点项目(98-2-178;99-2-193)

摘　　要：测定了从食用菌毛头鬼伞(Coprinuscomatus)中提纯的y3蛋白对烟草花叶病毒(Tobaccomosaicvirus,TMV)的钝化作用,结果表明y3对TMV有较强的体外钝化作用,在心叶烟枯斑寄主上对TMV的抑制中浓度约为2.0靏/mL;y3在pH9.0时较稳定;TMV与y3混合后用RNase处理,测得侵染率为61.74%,比未用RNase处理的对照降低了38.26%,说明y3具有一定的体外脱衣壳作用;另外电镜观察发现y3可使部分TMV粒体发生裂解,变短。A antiviral protein y3, from mushroom Coprinus comatus, could inactivate Tobacco mosaic virus (TMV) in vitro. The inhibition rate against is 50.0% when the concentration of y3 is 2.0靏/mL. When RNase was added to the mixture of TMV and y3, the infection rate of TMV was 61.74%, decreased by 38.26% in contrast with the control. It was also shown that y3 can break TMV particles when observed under electron microscope.

关 键 词：烟草花叶病毒 钝化 食用菌提取物 植物病毒 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]