PCR方法从进口饲料中检出转基因成分  

Transgenic ingredients identified in import feedstuffs by PCR

作　　者：闻伟刚[1] 贺水山[1] 杜华宏[1] 赵秀玲[1] 孙立群[1] 

机构地区：[1]宁波出入境检验检疫局,315012

出　　处：《植物检疫》2004年第1期8-10,共3页Plant Quarantine

摘　　要：本方法通过PCR扩增 ,从进口饲料泰农 - 40中得到 35S启动子和Nos终止子外源基因片段 ,同时 ,从进口饲料效美素中得到Nos终止子基因片段 ,经酶切验证 ,最后判定这两种饲料中均含有转基因成分。The 35S and Nos forgign genes were identified in Tainong-40 by PCR,and Nos gene was detected only in Xiaomeisu. Through the validate of endonuclease,this two sorts of import feedstuffs (Tainong-40 and Xiaomeisu) were determined to contain transgenic ingredients.

关 键 词：进口饲料 泰农-40 效美素 PCR 转基因成分 

分 类 号：S816[农业科学—饲料科学]