维生素C和E混合饲喂对中华鳖幼鳖抗酸应激能力的影响  

Effects of the Combination Feeding of Vitamin C and E on Anti-acid Stress Ability in Juvenile Soft-shelled Turtles (Pelodiscus sinensis)

作　　者：周显青[1] 牛翠娟[1] 孙儒泳[1] 

机构地区：[1]北京师范大学生命科学学院生物多样性与生态工程教育部重点实验室

出　　处：《Zoological Research》2004年第1期37-42,共6页动物学研究（英文）

摘　　要：来自甲鱼养殖场的 6 0只中华鳖 (Pelodiscussinensis)幼鳖驯养 3周后 ,实验设 5组 :对照组、处理Ⅰ、Ⅱ、Ⅲ和Ⅳ组。各组设 2个平行 ,依次在饵料中混合添加维生素C (Vc)和E (VE)为 0和 0、 2 5 0和 5 0、2 5 0 0和 5 0、 2 5 0和 2 5 0、 2 5 0 0和 2 5 0mg/kg ,喂食 4周后 ,每组取半数幼鳖经酸应激处理 2 4h。取幼鳖血液 ,用镜检法测定血细胞的吞噬率 ,透射比浊法测定血清溶菌活力、杀菌活力以及补体C3和C4含量。①经酸应激与未经酸应激处理相比 :对照组血细胞吞噬率显著降低 ,而处理Ⅰ～Ⅳ组无显著变化 ;对照组和处理Ⅰ组血清溶菌活力和补体C3含量显著下降 ,而处理Ⅱ～Ⅳ组无显著变化 ;血清杀菌活力均有显著下降 (对照组、处理Ⅰ和Ⅲ组极显著 ,处理Ⅱ组和Ⅳ组显著 ) ;对照组、处理Ⅰ和Ⅲ组血清补体C4显著下降 ,而处理Ⅱ和Ⅴ组无显著变化。②经酸应激处理 ,血细胞吞噬率、血清溶菌活力、杀菌活力和补体C3含量 ,处理Ⅰ～Ⅳ组的均显著高于对照组 ,处理Ⅳ组显著高于其他 4组 ;血清杀菌活力 ,处理Ⅱ组又高于处理Ⅰ和Ⅲ组 ;血清补体C4 ,对照组显著低于处理Ⅰ～Ⅳ组 ,而处理Ⅰ～Ⅳ组间无显著相异。Vc 和VE 混合饲喂对酸应激后中华鳖血细胞吞噬率、血清溶菌活力、杀菌活力和补体C3含量有显著协同促进作用 ,对?The 60 juvenile soft-shelled turtles (Pelodiscus sinensis) obtained from a commercial farm were acclimated in laboratory for three weeks.The five groups juvenile turtles [one control group (C) and four experimented groups (Ⅰ,Ⅱ,Ⅲ and Ⅳ)] were fed with V c and V E supplementation at the dosages of 0 and 0,250 and 50,2500 and 50,250 and 250,2 500 and 250mg/kg diets,respectively.There were two replicates per group.After feeding 4 weeks,half turtles per group were treat with acid-stress for 24 h.The blood in turtles were collected,and the percentage phagocytic rate of blood cell was analyzed by counting under microscope;serum bacteriolytic activity,bactericidal activity,levels of complement C3 and C4 were analyzed by transmission colorimetric method.The results showed:①the phagocytic rates of blood cell in stressed C significantly lowered compared with non-stressed C,while there were no significant changes in the other four groups of Ⅰ-Ⅳ between stressed and non-stressed turtles;serum bacteriolytic activity and the level of complement C3 in the stressed C and Ⅰ significantly declined,while there were no significant changes in Ⅱ-Ⅳ compared with non-stress groups.Serum bactericidal activity in all stressed groups lowered (the three groups of C,Ⅰ and Ⅲ tremendously significantly lowered,and Ⅱ and Ⅳ significantly lowered).The levels of complement C4 in the stressed three groups of C,Ⅰ and Ⅲ were significantly declined,while the other stressed two groups of Ⅱ and Ⅳ had no significant change compared with non-stressed ones.②Among of the acid stressed juvenile turtles,the phagocytic rate of blood cell,serum bacteriolytic activity,bactericidal activity and level of complement C3 in Ⅰ-Ⅳ were significantly higher than that in C,and Ⅳ was significantly higher than the other four groups.The bactericidal activity in Ⅱ was higher than both Ⅰ and Ⅲ.The level of complement C4 in C was significantly lower than those in Ⅰ-Ⅳ,while there was no significant difference among the four

关 键 词：维生素C 维生素E 酸应激 吞噬率 溶菌活力 杀菌活力 中华鳖 

分 类 号：S966.5[农业科学—水产养殖]